Expression of the nuclear encoded OEE1 protein is required for oxygen evolution and stability of photosystem II particles in Chlamydomonas reinhardtii.
Expression of the nuclear encoded OEE1 protein is required for oxygen evolution and stability of photosystem II particles in Chlamydomonas reinhardtii.
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DOI：
10.1002/j.1460-2075.1987.tb04756.x
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发表时间：
1987-02
期刊：
影响因子：
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通讯作者：
S. Mayfield;P. Bennoun;J. Rochaix
中科院分区：
文献类型：
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作者：
S. Mayfield;P. Bennoun;J. Rochaix
在莱茵衣藻中，放氧增强子蛋白1（OEE 1）是光系统II（PS II）放氧复合物的一部分，由单个核基因（psb 1）编码。核突变体FuD 44特别缺乏OEE 1多肽，并且完全缺乏光合放氧。在该突变体中，在psb 1基因的5′区域插入5 kb DNA导致OEE 1 mRNA和蛋白质完全缺失。回复突变体FuD 44-R 2能够进行野生型细胞30%的光合放氧，已经丢失了5 kb DNA插入片段中的4 kb，并积累了OEE 1 mRNA和蛋白质，尽管水平略低于野生型细胞。FuD 44突变体中OEE 1蛋白的缺失不影响其他核编码的PS II外周多肽的积累。然而，OEE 1的缺失确实导致叶绿体编码的PS II核心多肽的更快的周转，从而导致FuD 44细胞中PS II核心多肽的实质性缺乏。这些PS II核心蛋白再次在回复突变体FuD 44-R2细胞中积累。
In Chlamydomonas reinhardtii the oxygen evolving enhancer protein 1 (OEE1), which is part of the oxygen evolving complex of photosystem II (PS II), is coded for by a single nuclear gene (psb1). The nuclear mutant FuD44 specifically lacks the OEE1 polypeptide and is completely deficient in photosynthetic oxygen evolution. In this mutant a 5 kb DNA insertion into the 5′ region of the psb1 gene results in the complete absence of OEE1 mRNA and protein. A revertant, FuD44‐R 2, which is capable of 30% of the photosynthetic oxygen evolution of wild‐type cells, has lost 4 kb of the 5 kb DNA insert, and accumulates both OEE1 mRNA and protein, although at levels somewhat less than those of wild‐type cells. Absence of the OEE1 protein in the FuD44 mutant does not affect the accumulation of other nuclear encoded PS II peripheral polypeptides. OEE1 absence does, however, result in a more rapid turnover of the chloroplast encoded PS II core polypeptides, thus resulting in a substantial deficiency of PS II core polypeptides in FuD44 cells. These PS II core proteins again accumulate in revertant FuD44‐R2 cells.