Method of Designing Pre‐freezing Protocol in Cryopreservation of Biological Materials
Method of Designing Pre‐freezing Protocol in Cryopreservation of Biological Materials
复制标题
生物材料超低温保存预冻方案的设计方法
DOI：
10.1111/j.1749-6632.1998.tb10151.x
复制
发表时间：
1998
影响因子：
5.2
通讯作者：
I. Tanasawa
中科院分区：
文献类型：
--
作者：
R. Shirakashi;I. Tanasawa
摘要：讨论了生物材料低温保存中确定预冻方案的程序。为了冷冻保存生物材料，特别是具有大尺寸的组织，在预冷冻过程之后，冷冻保护剂（CPA）的浓度应该足够高，因为高浓度CPA溶液可以在相当低的冷却速率下玻璃化。在预冻过程中，CPA的浓度逐渐升高，以避免渗透应力。在本文中，提出了一种设计预冻结协议的常规方法。CPA浓度的增加速率可基于Kedem-Katchalsky方程使用膜渗透率Lp、w和s计算。最佳方案可以通过计算其中细胞的归一化体积在整个过程中保持恒定的过程来确定。
ABSTRACT: Procedures of determing the pre‐freezing protocol in cryopreservation of biological materials are discussed. In order to cryopreserve biological materials, especially tissues with large sizes, the concentration of cryoprotective agents (CPAs) should be high enough after pre‐freezing process, because a high concentration CPA solution can be vitrified a rather low cooling rate. In the pre‐freezing process, the concentration of CPA is raised gradually to avoid osmotic stress. In this paper, a conventional method of designing the pre‐freezing protocol is proposed. The rate of increase of the concentration of CPA can be calculated on the basis of Kedem‐Katchalsky equations using the membrane permeabilities, Lp, w and s. Optimal protocol can be determined by calculating a process in which the normalized volume of the cell is kept constant whole through the process.
影响因子：
2.7
作者：
FAHY, GM;MACFARLANE, DR;MERYMAN, HT
通讯作者：
MERYMAN, HT