RETRACTED ARTICLE: MicroRNA-32 inhibits osteosarcoma cell proliferation and invasion by targeting Sox9
RETRACTED ARTICLE: MicroRNA-32 inhibits osteosarcoma cell proliferation and invasion by targeting Sox9
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DOI：
10.1007/s13277-014-2229-x
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发表时间：
2014-07
期刊：
影响因子：
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通讯作者：
Jian-qiang Xu;Weibin Zhang;R. Wan;Yao Yang
中科院分区：
文献类型：
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作者：
Jian-qiang Xu;Weibin Zhang;R. Wan;Yao Yang
越来越多的研究表明microRNAs（miRNAs）的发现可能为包括骨肉瘤在内的人类肿瘤提供新的治疗靶点。先前的研究表明，miR-32在乳腺癌和子宫内膜癌中失调。然而，其在骨肉瘤中的生物学作用仍不清楚。在本研究中，我们发现miR-32在骨肉瘤组织中的表达明显低于癌旁正常组织。体外研究进一步证明，miR-32模拟物能够抑制Saos-2和U2 OS细胞的增殖，而其反义寡核苷酸促进细胞增殖。在分子水平上，我们的数据进一步揭示了Sox 9的表达受miR-32的负调控。因此，我们的研究结果确定了miR-32在骨肉瘤中通过调节Sox 9表达的重要作用。
Increasing reports suggest that discovery of microRNAs (miRNAs) might provide a novel therapeutical target for human cancers, including osteosarcoma. Previous studies have shown that miR-32 was dysregulated in breast and endometrial cancer. However, its biological roles in osteosarcoma remain unclear. In the current study, we found that miR-32 was significantly down-regulated in osteosarcoma tissues, compared with the adjacent normal tissues. In vitro studies further demonstrated that miR-32 mimics were able to suppress, while its antisense oligos promoted cell proliferation in Saos-2 and U2OS cells. At the molecular level, our data further revealed that expression of Sox9 was negatively regulated by miR-32. Therefore, our results identify an important role for miR-32 in the osteosarcoma through regulating Sox9 expression.