AN IMPROVED PHOTOCHEMICAL MODEL OF EMBOLIC CEREBRAL INFARCTION IN RATS
AN IMPROVED PHOTOCHEMICAL MODEL OF EMBOLIC CEREBRAL INFARCTION IN RATS
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DOI：
10.1161/01.str.22.2.225
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发表时间：
1991-02-01
期刊：
影响因子：
8.3
通讯作者：
FUTRELL, N
中科院分区：
文献类型：
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作者：
FUTRELL, N
为了提供进一步的证据，证明在大鼠颈动脉光化学损伤后发现的多发性脑梗死是由栓子引起的，并消除先前光化学栓塞中风模型（虎红和绿色激光）报告的全身性低血压和血液加热，我修改了光化学技术。 在注射光敏染料Photofrin II后，用红色激光（632 nm）以100至800 mW/cm 2的功率照射10或20分钟后，在18只Wistar大鼠的颈动脉中研究脑病理学。 多个脑小动脉被含有频繁红细胞和白细胞的血小板聚集体闭塞，与颈动脉中的血栓形成物质相同，但与虎红模型中颈动脉和脑中观察到的血小板聚集体不同。 80例梗死随机分布在非闭塞性颈动脉血栓同侧的整个大脑中。 只有在激光功率高于（1，600 mW/cm 2）或激光照射时间长于（25分钟）的情况下，血液才会显著加热（0.5 ℃或更高）。 该模型模拟了人类中风的一种机制，并提供了一种系统研究小脑梗死形态演变的方法。
To provide further evidence that the multiple cerebral infarcts found in rats following photochemical damage to the carotid artery are caused by emboli and to eliminate the systemic hypotension and heating of the blood reported with the previous photochemical embolic stroke model (rose bengal and a green laser), I have modified the photochemical technique. Brain pathology was studied in 18 Wistar rats following carotid artery irradiation with a red laser (632 nm) at powers ranging from 100 to 800 mW/cm2 for 10 or 20 minutes following the injection of the photosensitizing dye Photofrin II. Multiple cerebral arterioles were occluded by platelet aggregates containing frequent erythrocytes and leukocytes, identical to the thrombotic material in the carotid artery but different from the platelet aggregates seen in the carotid artery and the brain in the rose bengal model. Eighty infarcts were distributed randomly throughout the brain ipsilateral to the nonocclusive carotid thrombus. Significant heating (0.5-degrees-C or more) of the blood occurred only with laser powers higher (1,600 mW/cm2) or laser irradiations longer (25 minutes) than those used in the improved model of embolic stroke. This model mimics one mechanism of stroke in humans and provides a means to study systematically the morphological evolution of small cerebral infarcts.