Effect of disodium fumarate on microbial abundance, ruminal fermentation and methane emission in goats under different forage: concentrate ratios.
Effect of disodium fumarate on microbial abundance, ruminal fermentation and methane emission in goats under different forage: concentrate ratios.
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DOI：
10.1017/s1751731112000857
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发表时间：
2012-11
期刊：
影响因子：
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通讯作者：
C. J. Yang;S. Mao;L. Long;W. Zhu
中科院分区：
文献类型：
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作者：
C. J. Yang;S. Mao;L. Long;W. Zhu
研究了富马酸二钠（DF）对不同粗精比（F：C）和维持日粮条件下山羊甲烷排放、瘤胃发酵和微生物丰度的影响。采用4 × 4拉丁方试验设计，试验组采用2 × 2析因设计，主要因素为日粮饲粮比（41：59或58：42）和日粮膳食纤维添加量（0或10 g/d）。无论F：C比例如何，DF平均降低了11.9%的甲烷产量（P < 0.05）。日粮中添加DF后，山羊瘤胃内总挥发性脂肪酸、乙酸和丙酸的含量显著增加（P < 0.05），而甲烷菌的丰度显著降低（P < 0.05）。在高饲料日粮中，丰富的反刍月形单胞菌，一种减少脂肪酸的细菌，是更大的山羊瘤胃补充DF。真菌、原生动物、产黄瘤胃球菌和产琥珀酸纤维杆菌的丰度不受DF添加的影响。不同的F：C比例影响甲烷菌、真菌和R.产黄杆菌对甲烷排放无影响（P> 0.05）。结果表明，DF对饲喂不同F：C比日粮的山羊瘤胃内发酵均有促进作用，但这种促进作用并非直接作用于厌氧真菌、原生动物和纤维分解菌等纤维降解产氢微生物，而是通过刺激瘤胃内脂肪酸还原菌和抑制产甲烷菌而实现的。
This study investigated the effects of disodium fumarate (DF) on methane emission, ruminal fermentation and microbial abundance in goats under different forage (F) : concentrate (C) ratios and fed according to maintenance requirements. Four ruminally fistulated, castrated male goats were used in a 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments and the main factors being the F : C ratios (41 : 59 or 58 : 42) and DF supplementation (0 or 10 g/day). DF reduced methane production (P < 0.05) on average by 11.9%, irrespective of the F : C ratio. The concentrations of total volatile fatty acids, acetate and propionate were greater in the rumen of goats supplemented with DF (P < 0.05), whereas the abundance of methanogens was lower (P < 0.05). In high-forage diets, the abundance of Selenomonas ruminantium, a fumarate-reducing bacterium, was greater in the rumen of goats supplemented with DF. The abundance of fungi, protozoa, Ruminococus flavefaciens and Fibrobacter succinogenes were not affected by the addition of DF. Variable F : C ratios affected the abundance of methanogens, fungi and R. flavefaciens (P < 0.05), but did not affect methane emission. The result implied that DF had a beneficial effect on the in vivo rumen fermentation of the goats fed diets with different F : C ratios and that this effect were not a direct action on anaerobic fungi, protozoa and fibrolytic bacteria, the generally recognized fiber-degrading and hydrogen-producing microorganisms, but due to the stimulation of fumarate-reducing bacteria and the depression of methanogens.