Fabrication of gelatin nanofibrous scaffolds using ethanol/phosphate buffer saline as a benign solvent.
Fabrication of gelatin nanofibrous scaffolds using ethanol/phosphate buffer saline as a benign solvent.
复制标题
DOI：
10.1002/bip.22120
复制
发表时间：
2012-12
期刊：
影响因子：
2.9
通讯作者：
Zhengbao Zha;Weibing Teng;Valerie Markle;Z. Dai;Xiaoyi Wu
中科院分区：
文献类型：
--
作者：
Zhengbao Zha;Weibing Teng;Valerie Markle;Z. Dai;Xiaoyi Wu
用于生物医学应用的天然聚合物纳米纤维的静电纺丝通常需要使用细胞毒性有机溶剂。在这项研究中，明胶纳米纤维静电纺丝从磷酸盐缓冲盐水/乙醇二元混合物作为一种良性溶剂在环境温度下。分析了离子强度、乙醇浓度和明胶浓度对明胶溶液可纺性和纤维微结构的影响。静电纺丝支架在乙醇中与戊二醛气相交联期间保持其形态，随后通过水冲洗除去纳米纤维中所含的盐。当完全水合时，机械预处理的支架显示25.5 ± 5.3kPa的杨氏模量、55.5 ± 13.9kPa的拉伸强度、160 ± 15%的变形性和89.9 ± 1.8%的回弹性。当在明胶支架上培养时，3 T3成纤维细胞显示出梭形形态，类似于细胞在3D细胞外基质中的正常形态。
Electrospinning of natural polymer nanofibers useful for biomedical applications often requires the use of cytotoxic organic solvents. In this study, gelatin nanofibers are electrospun from phosphate buffer saline/ethanol binary mixtures as a benign solvent at ambient temperature. The influences of ionic strength, ethanol concentration, and gelatin concentration on the electrospinnability of gelatin solutions and the fiber microarchitectures are analyzed. The electrospun scaffolds retain their morphologies during vapor-phase crosslinking with glutaraldehyde in ethanol and the subsequent removal of salts contained in the nanofibers via water rinsing. When fully hydrated, the mechanically preconditioned scaffolds display a Young's modulus of 25.5 ± 5.3 kPa, tensile strength of 55.5 ± 13.9 kPa, deformability of 160 ± 15%, and resilience of 89.9 ± 1.8%. When cultured on the gelatin scaffolds, 3T3 fibroblasts displayed spindle-like morphology, similar to the cell's normal morphology in a 3D extracellular matrix.