Actions of the testicular paracrine factor (P-Mod-S) on Sertoli cell transferrin secretion throughout pubertal development.
Actions of the testicular paracrine factor (P-Mod-S) on Sertoli cell transferrin secretion throughout pubertal development.
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DOI：
10.1210/endo-129-1-353
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发表时间：
1991-07
期刊：
影响因子：
4.8
通讯作者：
C. T. Anthony;M. Rosselli;M. Skinner
中科院分区：
文献类型：
--
作者：
C. T. Anthony;M. Rosselli;M. Skinner
围绕生精小管的管周细胞已被证明产生一种旁分泌因子，称为P-Mod-S，其在体外对支持细胞功能具有显著影响，并且被假定在睾丸功能的控制中是重要的。本研究旨在确定P-Mod-S是否具有调节青春期发育期间支持细胞功能的能力。从10-，20-，和35日龄的大鼠，对应于青春期前，青春期中期，和青春期后期发育阶段分离支持细胞。对从每个年龄组分离的培养细胞进行组织化学分析，以确定所用细胞群的纯度。睾丸转铁蛋白生产支持细胞被用作细胞分化的标志物。基础生产的转铁蛋白的培养细胞被发现在青春期增加。P-Mod-S刺激从10日龄、20日龄和35日龄大鼠分离的支持细胞产生转铁蛋白。FSH似乎可以增强支持细胞对10日龄大鼠细胞P-Mod-S的反应能力。35日龄大鼠的支持细胞对FSH等调节剂无反应。然而，P-Mod-S单独显着刺激转铁蛋白的生产支持细胞从这个更成熟的发展阶段。P-Mod-S是测试的唯一可以刺激35日龄大鼠支持细胞产生转铁蛋白的个体调节剂。结果表明，P-Mod-S能够在整个青春期发育过程中调节支持细胞功能。观察表明，P-Mod-S和FSH可能共同作用于青春期前睾丸，以促进支持细胞分化，P-Mod-S可能在成年睾丸中起作用，以维持最佳的支持细胞功能和分化。
Peritubular cells that surround the seminiferous tubules have been shown to produce a paracrine factor, termed P-Mod-S, that has dramatic effects on Sertoli cell function in vitro and is postulated to be important in the control of testicular function. The current study was designed to determine whether P-Mod-S has the ability to regulate Sertoli cell function during pubertal development. Sertoli cells were isolated from 10-, 20-, and 35-day-old rats which correspond to the prepubertal, mid-pubertal, and late pubertal stages of development. Histochemical analysis of cultured cells isolated from each age group was performed to establish the purity of the cell populations used. Testicular transferrin production by Sertoli cells was used as a marker of cellular differentiation. Basal production of transferrin by the cultured cells was found to increase during the pubertal period. P-Mod-S stimulated transferrin production by Sertoli cells isolated from 10-, 20-, and 35-day-old rats. FSH appears to enhance the ability of Sertoli cells to respond to P-Mod-S with cells obtained from 10-day-old rats. Sertoli cells from 35-day-old rats were nonresponsive to regulatory agents such as FSH. P-Mod-S alone, however, significantly stimulated transferrin production by Sertoli cells from this more adult stage of development. P-Mod-S was the only individual regulatory agent tested that could stimulate transferrin production by Sertoli cells from 35-day-old rats. Results indicate that P-Mod-S has the ability to regulate Sertoli cell function throughout pubertal development. Observations suggest that P-Mod-S and FSH may act together in the prepubertal testis to promote Sertoli cell differentiation and that P-Mod-S may act in the adult testis to maintain optimal Sertoli cell function and differentiation.