The kinesin Eg5 drives poleward microtubule flux in Xenopus laevis egg extract spindles.
The kinesin Eg5 drives poleward microtubule flux in Xenopus laevis egg extract spindles.
复制标题
驱动蛋白EG5在Xenopus laevis鸡蛋提取物中驱动极前微管通量。
DOI：
10.1083/jcb.200407126
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发表时间：
2004-12-06
影响因子：
7.8
通讯作者：
Mitchison, Timothy J
中科院分区：
文献类型：
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作者：
Miyamoto, David T;Perlman, Zachary E;Burbank, Kendra S;Groen, Aaron C;Mitchison, Timothy J
虽然有丝分裂和减数分裂纺锤体在中期保持稳定的长度，但它们的反平行微管以恒定的速率向纺锤体极点滑动。这种微管的“极向流动”发生在许多生物体中，可能为染色体分离提供了部分动力。我们采用定量图像分析的方法，研究了卵泡激蛋白Eg5在非洲爪蟾卵提取液中期纺锤体向极通量中的作用。药物抑制Eg5导致剂量反应性通量减慢，生化耗竭Eg5显著降低通量速率。我们的研究结果表明，非进程Eg5电机的集合驱动了中期爪蟾提取物纺锤体的通量。
Although mitotic and meiotic spindles maintain a steady-state length during metaphase, their antiparallel microtubules slide toward spindle poles at a constant rate. This “poleward flux” of microtubules occurs in many organisms and may provide part of the force for chromosome segregation. We use quantitative image analysis to examine the role of the kinesin Eg5 in poleward flux in metaphase Xenopus laevis egg extract spindles. Pharmacological inhibition of Eg5 results in a dose–responsive slowing of flux, and biochemical depletion of Eg5 significantly decreases the flux rate. Our results suggest that ensembles of nonprocessive Eg5 motors drive flux in metaphase Xenopus extract spindles.