Evaluation of variation of root shape of Japanese radish (Raphanus sativus L.) based on image analysis using elliptic Fourier descriptors
Evaluation of variation of root shape of Japanese radish (Raphanus sativus L.) based on image analysis using elliptic Fourier descriptors
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DOI：
10.1023/a:1018392531226
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发表时间：
1998-01-01
期刊：
影响因子：
1.9
通讯作者：
Ukai, Y
中科院分区：
文献类型：
--
作者：
Iwata, H;Niikura, S;Ukai, Y
利用基于椭圆傅立叶描述子的主成分得分对日本萝卜的根形变异进行了定量评价，分析了不同品种、不同土壤类型和不同生育阶段的差异。将35 mm彩色反转胶片上的取样根的摄影图像转换为数字图像。经过图像处理后，每个根部的轮廓用链码表示，然后用77个系数的椭圆傅立叶描述子来描述。在对轮廓的大小、旋转和起始点进行归一化后，由于人为确定的根曲率方向可能会影响结果，因此分别分析了与形状的对称和非对称变化相关的两组系数。系数的主成分分析表明，对称性(A)和非对称性(B)变异的大部分最多由5个成分构成。累计贡献率分别为95.2%和97.1%。各主成分的方差分析表明，由A组系数得到的第1、2、3主成分的品种效应达到极显著水平，它们分别与根长宽比、根远端钝度、根中部膨大有关。这表明这些性状是可遗传的，可以通过基于本报告中提出的椭圆傅立叶描述符的量化措施进行有效选择。根的弯曲方向和程度可以独立于对称性变化进行分析。
Variation was of root shape in Japanese radish, due to genotypes, soil types and growth stages, were quantitatively evaluated by principal components scores based on elliptic Fourier descriptors. Photographic images of sampled roots on 35mm color reversal films were converted into digital images. After image processing, the contour of each root was expressed as chain-code and then described by 77 coefficients of elliptic Fourier descriptors. After normalization about size, rotation, and starting point of the contour, two groups of the coefficients, which are related to the symmetrical and asymmetrical variations of shape, were analyzed separately, since artificially determined direction of curvature of the root may influence the results. Principal component analysis of the coefficients showed that the major part of the symmetrical (A) and asymmetrical (B) variations were summarized by at most 5 components. The cumulative contribution was 95.2% and 97.1%, respectively. Analysis of variance of each component indicated that the variety effect was highly significant for the Ist, 2nd and 3rd principal components derived from group A coefficients, which were related to the aspect ratio, bluntness of the distal part of the root, and swelling of the middle part, respectively. This suggests that these traits are heritable and can be effectively selected through quantified measures based on elliptic Fourier descriptors presented in this report. Direction and degree of curvature of root could be analyzed independently of the symmetrical variation.