Detection of non-primate hepaciviruses in UK dogs
Detection of non-primate hepaciviruses in UK dogs
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DOI：
10.1016/j.virol.2015.05.005
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发表时间：
2015-10-01
期刊：
影响因子：
3.7
通讯作者：
Brownlie, J.
中科院分区：
文献类型：
--
作者：
El-Attar, L. M. R.;Mitchell, J. A.;Brownlie, J.
非灵长类肝炎病毒（NPHV）已在狗，马，蝙蝠和野生啮齿动物中发现。然而，在美国以外的狗中存在NPHV尚未确定。在这里，我们首次描述了在英国犬群中检测到NPHV（在整个手稿中描述为CnNPHV）。我们检查了从安置在一个重新归巢的狗舍中的狗收集的组织，其中呼吸道疾病是地方性的。通过RT-PCR在48/210只狗的气管组织中检测到CnNPHV RNA，并且在12/20只狗的肝脏、肺和/或气管组织中检测到CnNPHV RNA。通过原位杂交证实了CriNPHV RNA的存在及其向性。组织病理学检查表明，在CnNPHV RNA阳性的呼吸道组织中，组织病理学评分有升高的趋势，尽管这在统计学上并不显著。我们的发现拓宽了CnNPHV的地理分布和我们对它的理解。CnNPHV在犬科动物中复制的进一步证据值得调查。(C)由Elsevier Inc.出版。
Non-primate hepacivirus (NPHV) has been identified in dogs, horses, bats and wild rodents. The presence of NPHV in dogs outside of the USA however is yet to be established. Here we describe for the first time the detection of NPHV in the UK dog population (described throughout the manuscript as CnNPHV). We examined tissues collected from dogs housed in a rehoming kennel where respiratory disease was endemic CnNPHV RNA was detected in the tracheal tissues of 48/210 dogs by RT-PCR, and in the liver, lung and/or tracheal tissues of 12/20 dogs. The presence of CriNPHV RNA, and its tropism was confirmed by in situ hybridisation. Histopathological examination demonstrated a trend toward higher histopathological scores in CnNPHV RNA positive respiratory tissues, although, this was not statistically significant Our findings broaden the geographic distribution and our understanding of CnNPHV. Further evidence of CnNPHV replication in canids warrants investigation. (C) 2015 Published by Elsevier Inc.