Visfatin reduces hippocampal CA1 cells death and improves learning and memory deficits after transient global ischemia/reperfusion
Visfatin reduces hippocampal CA1 cells death and improves learning and memory deficits after transient global ischemia/reperfusion
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DOI：
10.1016/j.npep.2014.12.004
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发表时间：
2015-02-01
期刊：
影响因子：
2.9
通讯作者：
Gorjipour, Fazel
中科院分区：
文献类型：
--
作者：
Erfani, Sohaila;Khaksari, Mehdi;Gorjipour, Fazel
Visfatin是一种新型的具有胰岛素模拟作用的脂肪细胞因子，它参与胰岛素的降糖作用，提高胰岛素敏感性。它与多种细胞过程有关，在细胞凋亡和存活中起重要作用。此外，脑缺血导致海马锥体细胞的损失，因此，在本研究中；研究了visfatin对雄性大鼠全脑缺血后的神经保护作用。双颈总动脉闭塞20分钟，再灌注4天。动物在再灌注时分别给予Visfatin（脑室内；100 ng）或生理盐水（2 μ l）。缺血4天后进行行为学检查、细胞凋亡和坏死评估。Visfatin显著降低海马CA1区Caspase-3活化（P < 0.001）、TUNEL阳性细胞（P < 0.05）和坏死细胞死亡（P < 0.001）。visfatin可显著改善脑缺血再灌注大鼠的记忆缺陷（P < 0.05）。结果表明，内脏素通过其抗凋亡特性对大鼠脑缺血再灌注损伤具有明显的神经保护作用。(C) 2014 Elsevier Ltd.版权所有。
Visfatin is a novel adipocytokine with insulin-mimetic effect which plays a role in glucose-lowering effect of insulin and improves insulin sensitivity. It has been linked to a variety of cellular processes and its plays important roles in cell apoptosis and survival. Moreover, cerebral ischemia causes loss of hippocampus pyramidal cells, therefore, in this study; we investigated the neuroprotective effect of visfatin after global cerebral ischemia in male rats. Both common carotid arteries were occluded for 20 minutes followed by 4 days of reperfusion. Animals were treated with either the Visfatin (intracerebroventricular; 100 ng) or saline vehicle (2 mu l) at the time of reperfusion. Behavioral examination, apoptosis and necrosis assessment were performed 4 days after ischemia. Visfatin significantly reduced Caspase-3 activation (P < 0.001), TUNEL positive cells (P < 0.05) and necrotic cell death in the CA1 region of the hippocampus (P < 0.001). Moreover, treatment with visfatin significantly improved memory deficits of cerebral ischemia-reperfusion rats (P < 0.05). The results suggest that visfatin via its antiapoptotic properties has significant neuroprotective effects on cerebral ischemia reperfusion injury in rats. (C) 2014 Elsevier Ltd. All rights reserved.