Beneficial Storage Effects of Epigallocatechin-3-O-Gallate on the Articular Cartilage of Rabbit Osteochondral Allografts
Beneficial Storage Effects of Epigallocatechin-3-O-Gallate on the Articular Cartilage of Rabbit Osteochondral Allografts
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DOI：
10.1177/096368970901805-604
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发表时间：
2009-01-01
影响因子：
3.3
通讯作者：
Hyon, Suong-Hyu
中科院分区：
文献类型：
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作者：
Bae, Jung Yoon;Matsumura, Kazuaki;Hyon, Suong-Hyu
新鲜骨软骨移植是治疗膝关节软骨缺损最有效的方法之一。尽管在临床上取得了成功，但是新鲜的同种异体骨软骨移植存在很大的局限性，因为软骨组织保存时间短，不能很好地保存。新鲜骨软骨移植物一般在4℃的培养基中保存，而在培养基中保存的关节软骨的活力在1周内明显下降，适当的血清学检测以减少疾病传播的机会至少需要2周。（-）-表没食子儿茶素-3- o -没食子酸酯（EGCG）对癌细胞和正常细胞的增殖具有不同的作用，因此对各种癌细胞具有细胞毒性作用，但对正常细胞具有细胞保留作用。因此，含有EGCG的储存液可以延长关节软骨的保存时间。将兔骨软骨移植于含EGCG的培养基中，4℃保存2周，4周后通过肉眼观察和组织学检查临床效果。EGCG保存的骨软骨移植物的软骨结构保存良好，细胞活力高，细胞外基质（ECM）中糖胺聚糖（GAG）含量高。同种异体骨软骨移植后，储存EGCG的骨软骨移植物也提供了更好的关节软骨保留，具有活力和代谢活性。这些数据表明，EGCG可以作为一种有效的储存剂，允许在冷藏条件下长期保存关节软骨。
A fresh osteochondral allograft is one of the most effective treatments for cartilage defects of the knee. Despite the clinical success, fresh osteochondral allografts have great limitations in relation to the short storage time that cartilage tissues can be well-preserved. Fresh osteochondral grafts are generally stored in culture medium at 4 degrees C. While the viability of articular cartilage stored in culture medium is significantly diminished within I week, appropriate serology testing to minimize the chances for the disease transmission requires a minimum of 2 weeks. (-)-Epigallocatechin-3-O-gallate (EGCG) has differential effects on the proliferation of cancer and normal cells, thus a cytotoxic effect on various cancer cells, but a cytopreservative effect on normal cells. Therefore, a storage solution containing EGCG might extend the storage duration of articular cartilages. Rabbit osteochondral allografts were performed with osteochondral grafts stored at 4 degrees C in culture medium containing EGCG for 2 weeks and then the clinical effects were examined with macroscopic and histological assessment after 4 weeks. The cartilaginous structure of an osteochondral graft stored with EGCG was well-preserved with high cell viability and glycosaminoglycan (GAG) content of the extracellular matrix (ECM). After an osteochondral allograft, the implanted osteochondral grafts stored with EGCG also provided a significantly better retention of the articular cartilage with viability and metabolic activity. These data suggest that EGCG can be an effective storage agent that allows long-term preservation of articular cartilage under cold storage conditions.