IgG deposition and activation of the classical complement pathway involvement in the activation of human granulocytes by decellularized porcine heart valve tissue
IgG deposition and activation of the classical complement pathway involvement in the activation of human granulocytes by decellularized porcine heart valve tissue
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DOI：
10.1016/j.biomaterials.2008.01.005
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发表时间：
2008-04-01
期刊：
影响因子：
14
通讯作者：
Weigel, Guenter
中科院分区：
文献类型：
--
作者：
Bastian, Fabienne;Stelzmueller, Marie-Elisabeth;Weigel, Guenter
心脏瓣膜的脱细胞处理被认为可以消除组织免疫原性。组织工程异种心脏瓣膜的早期失败见于儿童，这是这一有前途的研究领域的一个主要缺点。本研究旨在探讨脱细胞猪心脏瓣膜组织对体外免疫激活的影响。脱细胞猪组织与人血浆孵育导致IgG的吸附、经典补体途径的激活和活化的多形核白细胞（PMN）的粘附。这种炎症反应被从天然猪组织中提取的蛋白质强烈抑制，这可能表明存在于细胞外基质（ECM）中的PMN活化的抑制剂在去细胞化过程中丢失。(C)2008爱思唯尔有限公司保留所有权利。
Decellularization treatment of heart valves has been thought to eliminate tissue immunogenicity. Early failure of tissue-engineered xenogeneic heart valves was seen in children and has been a major drawback in this promising field of research. This study was designed to characterize the effects of acellular porcine heart valve tissue on immune activation in vitro. Incubation of decellularized porcine tissue with human plasma led to adsorption of IgG, activation of the classical complement pathway and adhesion of activated polymorphonuclear leukocytes (PMN). This inflammatory response was strongly inhibited by proteins extracted from native porcine tissue which might indicate that inhibitors of PMN activation present in the extracellular matrix (ECM) are lost during the decellularization process. (C) 2008 Elsevier Ltd. All rights reserved.