Decreased incidence of gap junctions between Chinese hamster V-79 cells upon exposure to the tumor promoter 12-O-tetradecanoyl phorbol-13-acetate.
Decreased incidence of gap junctions between Chinese hamster V-79 cells upon exposure to the tumor promoter 12-O-tetradecanoyl phorbol-13-acetate.
复制标题
暴露于肿瘤促进剂 12-O-十四烷酰佛波醇-13-乙酸酯后，中国仓鼠 V-79 细胞之间间隙连接的发生率降低。
DOI：
10.1016/0014-4827(82)90257-9
复制
发表时间：
1982
影响因子：
3.7
通讯作者：
Revel,JP
中科院分区：
文献类型：
--
作者：
Yancey,SB;Edens,JE;Trosko,JE;Chang,CC;Revel,JP
先前的研究表明，中国仓鼠V-79细胞之间的代谢合作被抑制的肿瘤促进佛波醇酯，但不是由那些佛波醇酯在肿瘤促进无效。代谢合作被认为是介导的间隙连接。因此，在本研究中，我们使用冷冻断裂和定量形态学技术来检查最有效的促肿瘤佛波酯，12-O-十四烷酰基佛波醇-13-乙酸酯（TPA）和无活性类似物，4α-佛波醇-12，13-二癸酸酯（4α-PDD）对V-79细胞之间缝隙连接接触频率的影响。在对照和4α-PDD处理的培养物中，连接频繁，尽管尺寸相当小。相比之下，在TPA处理的文化，缝隙连接很少，和由缝隙连接所占据的膜面积减少了20倍以上，从对照组中发现。这些结果表明，TPA诱导的V-79细胞之间的代谢合作的抑制是间隙连接的数量减少的结果。
Previous studies have shown that metabolic cooperation between Chinese hamster V-79 cells is inhibited by the tumor-promoting phorbol esters, but not by those phorbol esters inactive in tumor promotion. Metabolic cooperation is believed to be mediated by gap junctions. Therefore, in the present study we have used freeze-fracture and quantitative morphological techniques to examine the effect of the most potent tumor-promoting phorbol ester, 12-O-tetradecanoyl phorbol-13-acetate (TPA) and of the inactive analog, 4α-phorbol-12,13-didecanoate (4α-PDD) on the frequency of gap-junctional contacts between V-79 cells. In both control and in 4α-PDD treated cultures the junctions were frequent, though rather small in size. In contrast, in the TPA-treated cultures, gap junctions were few, and the area of membrane occupied by gap junctions was reduced more than 20-fold from that found for controls. These results suggest that the TPA-induced inhibition of metabolic cooperation between V-79 cells is a consequence of a decrease in the number of gap junctions.