Comparative infectivity of Babesia divergens and a zoonotic Babesia divergens-like parasite in cattle
Comparative infectivity of Babesia divergens and a zoonotic Babesia divergens-like parasite in cattle
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DOI：
10.4269/ajtmh.2005.73.865
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发表时间：
2005-11-01
影响因子：
3.3
通讯作者：
Goff, WL
中科院分区：
文献类型：
--
作者：
Holman, PJ;Spencer, AM;Goff, WL
在密苏里州和肯塔基州的人类巴贝斯虫病病例中以及在马萨诸塞州的南图克特岛的东部棉尾兔（Sylvilagus floridanits）中鉴定出的巴贝斯虫分歧样寄生虫具有相同的小亚基核糖体RNA基因序列。该序列与分歧巴伯螨的序列有99.8%的相同性，表明美国寄生虫可能是B。分歧菌，一种欧洲人和牛巴贝虫病的病原体。用培养的南图克特岛巴氏巴氏杆菌（NR 831）和B接种荷斯坦-弗里斯兰小牛。分歧寄生虫，并通过临床体征，Giemsa染色血膜，PCR和培养监测。NR 831受体没有表现出感染的临床体征，并且对于所有测定保持阴性。B。divergens受体发生临床感染，并通过所有测定变为阳性。NR 831受体对B攻毒完全敏感。分歧者本研究证实，南图克特岛Babysp.是不是同种与B。分歧基于牛的宿主特异性。
Babesia divergens-like parasites identified in human babesiosis cases in Missouri and Kentucky and in eastern cottontail rabbits (Sylvilagus floridanits) on Nantucket Island, Massachusetts, share identical small subunit ribosomal RNA gene sequences. This sequence is 99.8% identical to that of Babesia divergens, suggesting that the U.S. parasite may be B. divergens, a causative agent of human and bovine babesiosis in Europe. Holstein-Friesian calves were inoculated with cultured Nantucket Island Babesia sp. (NR831) and B. divergens parasites and monitored by clinical signs, Giemsa-stained blood films, PCR, and culture. The NR831 recipients did not exhibit clinical signs of infection and remained negative for all assays. The B. divergens recipients developed clinical infections and became positive by all assays. NR831 recipients were fully susceptible upon challenge inoculation with B. divergens. This study confirms that the Nantucket Island Babesia sp. is not conspecific with B. divergens based on host specificity for cattle.