Nicotinamide phosphoribosyltransferase (NAMPT/PBEF/visfatin) is constitutively released from human hepatocytes
Nicotinamide phosphoribosyltransferase (NAMPT/PBEF/visfatin) is constitutively released from human hepatocytes
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DOI：
10.1016/j.bbrc.2009.11.066
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发表时间：
2010-01-01
影响因子：
3.1
通讯作者：
Gebhardt, R.
中科院分区：
文献类型：
--
作者：
Garten, A.;Petzold, S.;Gebhardt, R.
循环NAMPT（PBEF/内脂素）具有多效性功能，由脂肪细胞分泌。由于血清中NAMPT水平是否可以单独由脂肪细胞分泌来解释尚不清楚，因此我们询问肝细胞是否也能够释放NAMPT。使用HepG 2细胞以及原代大鼠和人肝细胞，发现NAMPT向细胞培养上清液中的释放以时间依赖性方式组成性发生。在原代人肝细胞中，24小时内的分泌远远高于细胞内容物，但既不受分泌抑制剂的影响，也不受葡萄糖的影响。胰岛素或TNF α。通过尺寸排阻色谱法测定。HepG 2裂解物和上清液主要含有NAMPT的二聚体形式，其表现出类似的体外特异性酶活性。在原代人肝细胞中，分泌的NAMPT活性较低。我们的结果表明，人肝细胞是循环NAMPT的潜在来源。All rights reserved.
Circulating NAMPT (PBEF/visfatin) has pleiotropic functions and is secreted from adipocytes. Since it is doubtful whether serum levels can be explained by secretion from adipocytes alone, we asked whether hepatocytes are also able to liberate NAMPT. Using HepG2 cells and primary rat and human hepatocytes, release of NAMPT into the cell Culture Supernatant was found to Occur Constitutively in a time-dependent manner. In primary human hepatocytes, secretion within 24 h was far higher than the cellular content, but was neither influenced by inhibitors of secretion nor by glucose. insulin or TNF alpha. As determined by size exclusion chromatography. HepG2 lysates and supernatants primarily contained the dimeric form of NAMPT which exhibited similar in vitro specific enzymatic activity In primary human hepatocytes, secreted NAMPT was less active Our results demonstrate that human hepatocytes are a potential Source of circulating NAMPT (C) 2009 Elsevier Inc. All rights reserved.