Gas-liquid chromatography of mycotoxins of the trichothecene group.
Gas-liquid chromatography of mycotoxins of the trichothecene group.
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单端孢霉烯族霉菌毒素的气相色谱分析。
DOI：
10.1016/0003-2697(71)90261-2
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发表时间：
1971
影响因子：
2.9
通讯作者：
F. Strong
中科院分区：
文献类型：
--
作者：
C. Ikediobi;I. Hsu;J. R. Bamburg;F. Strong
本文介绍了一种用气相色谱法分离鉴定几种倍半萜类化合物的方法。含有游离羟基的化合物被色谱分离为三甲基甲硅烷基（TMS）醚，而天然存在的酯，如喜树碱直接色谱分离。通过等温和程序升温操作测定13种不同的异丙基乙烯的亚甲基单元值，对各个峰进行定性鉴定。使用葡萄糖TMS醚作为内标物提供了可用于定量测定多烯类化合物的相对响应因子。该方法已成功地应用于已知的混合物的纯化合物和T-2和HT-2毒素的测定粮食样品感染镰刀菌tricinctum。
A gas chromatography method for separation and identification of several trichothecenes is described. Compounds containing free hydroxyl groups were chromatographed as trimethylsilyl (TMS) ethers, while naturally occurring esters such as trichothecin were chromatographed directly. Qualitative identification of individual peaks was provided by determining the methylene unit values of each of 13 different trichothenes by both isothermal and temperature-programmed operation. Use of glucose TMS ether as an internal standard provided relative response factors useful in the quantitative determination of trichothecenes. The method was successfully applied to known mixtures of the pure compounds and to the determination of T-2 and HT-2 toxins in grain samples infected with Fusarium tricinctum.