Protease analysis by zymography: a review on techniques and patents.
Protease analysis by zymography: a review on techniques and patents.
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通过酶谱法进行蛋白酶分析：技术和专利综述。
DOI：
10.2174/187220809789389162
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发表时间：
2009
影响因子：
--
通讯作者：
L. Kurz
中科院分区：
文献类型：
--
作者：
J. Wilkesman;L. Kurz
酶谱法，一种在凝胶电泳上检测酶活性的技术，至少在过去的50年里已经在文献中被描述。虽然已经检测到各种各样的酶，特别是蛋白酶，但与其他分子生物学、生物技术和色谱技术相比，进展和改进速度较慢。大多数的评论和专利都集中在作为酶检测的一个元素的技术上，但详细的分析研究很少。涉及酶谱法本身的专利很少，技术本身在许多科学出版物的标题或关键词中几乎不是一个重要问题。这篇综述涵盖了迄今为止发表的关于电泳凝胶载体中蛋白水解酶的鉴定及其变体（如二维酶谱法、实时酶谱法和原位酶谱法）的一小部分研究成果。此外，将给出一个范围来可视化这种方法的新趋势，关于底物的使用和活性可视化。本文还探讨了酶谱学在不久的将来的发展前景。
Zymography, the detection of enzymatic activity on gel electrophoresis, has been a technique described in the literature for at least in the past 50 years. Although a diverse amount of enzymes, especially proteases, have been detected, advances and improvements have been slower in comparison with other molecular biology, biotechnology and chromatography techniques. Most of the reviews and patents published focus on the technique as an element for enzymatic testing, but detailed analytical studies are scarce. Patents referring to zymography per se are few and the technique itself is hardly an important issue in titles or keywords in many scientific publications. This review covers a small condensation of the works published so far dealing with the identification of proteolytic enzymes in electrophoretic gel supports and its variations like 2-D zymography, real-time zymography, and in-situ zymography. Moreover, a scope will be given to visualize the new tendencies of this method, regarding substrates used and activity visualization. What to expect from zymography in the near future is also approached.
用于微生物蛋白酶检测和表征的酶谱技术。
影响因子：
--
作者：
Lantz,MS;Ciborowski,P
通讯作者：
Ciborowski,P