PHOTODYNAMIC EFFECTS OF ROSE-BENGAL OR RIBOFLAVIN ON CARRIER-MEDIATED TRANSPORT-SYSTEMS IN RAT LENS
PHOTODYNAMIC EFFECTS OF ROSE-BENGAL OR RIBOFLAVIN ON CARRIER-MEDIATED TRANSPORT-SYSTEMS IN RAT LENS
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DOI：
10.1016/s0014-4835(81)80025-5
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发表时间：
1981-01-01
影响因子：
3.4
通讯作者：
KINOSHITA, JH
中科院分区：
文献类型：
--
作者：
JERNIGAN, HM;FUKUI, HN;KINOSHITA, JH
透镜会受到光敏剂和光的氧化损伤。在培养的大鼠晶状体中研究了核黄素或玫瑰红对载体介导的转运系统的光敏损伤。光氧化减少了86Rb、[3H]胆碱和[3H] α的积累。氨基异丁酸（AIB）在透镜水中。最早的影响是减少胆碱积累。其次是AIB和86 Rb的摄取减少，最后是86 Rb漏出增加和透明度丧失。核黄素或虎红对透镜转运的损害取决于光线和空气。超氧化物歧化酶（SOD）不能阻断两种光敏剂的光氧化作用，表明超氧化物自由基不是透镜损伤的直接原因。过氧化氢酶阻断了核黄素的作用，但没有阻断虎红的作用。相反，抗坏血酸阻断了虎红的作用，但仅部分阻断了核黄素的作用。因此，似乎核黄素通过过氧化氢中间体起作用，而玫瑰红通过其他中间体起作用，可能是单线态氧。
The lens is subject to oxidative damage by photosensitizers and light. Riboflavine or rose bengal photosensitized damage to carrier mediated transport systems was studied in cultured rat lenses. Photo-oxidation reduced accumulation of 86Rb, [3H]choline, and [3H].alpha.-aminoisobutyric acid (AIB) in the lens water. The earliest effect was decreased choline accumulation. This was followed by decreased uptake of AIB and 86Rb, and finally by increased 86Rb leakout and loss of clarity. Damage to lens transport by riboflavin or rose bengal was dependent upon light and air. Superoxide dismutase (SOD) did not block the photo-oxidation by either photosensitizer, indicating that the superoxide free radical was not directly responsible for the lens damage. Catalase blocked the effects of riboflavin but not of rose bengal. In contrast, ascorbate blocked the effects of rose bengal but only partially blocked the effects of riboflavin. There fore it appears that riboflavine acts through a hydrogen peroxide intermediate whereas rose bengal acts through some other intermediate, possibly singlet oxygen.