Identification of centromere regions in chromosomes of a unicellular red alga, Cyanidioschyzon merolae
Identification of centromere regions in chromosomes of a unicellular red alga, Cyanidioschyzon merolae
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单细胞红藻 Cyanidioschyzon merolae 染色体着丝粒区域的鉴定
DOI：
10.1016/j.febslet.2015.04.009
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
and Kan Tanaka
中科院分区：
文献类型：
--
作者：
Yu Kanesaki;Sousuke Imamura;Motomichi Matsuzaki;and Kan Tanaka
为了研究植物细胞中着丝粒结构的进化，鉴定原始藻类（如Cyanidioschyzonmerolae）的着丝粒区域是非常重要的。在先前的基因组计划中，计算机分析预测每条染色体中富含AT的区域为推定的着丝粒区域。在这里，我们通过使用抗CENP-A抗体的ChIP芯片分析确定了每条染色体中的着丝粒位置。鉴定出的着丝粒属于区域类型，长度约2-3 kb，不含共有元件或重复元件。原始真核植物细胞中的着丝粒可能起源于这些区域型着丝粒。
To investigate the evolution of centromere architecture in plant cells, it is important to identify centromere regions of primitive algae, such asCyanidioschyzon merolae. In a previous genome project, in silico analysis predicted an AT-rich region in each chromosome as putative centromere regions. Here, we identified a centromere position in each chromosome by ChIP-on-chip analysis using an anti-CENP-A antibody. The identified centromeres were of the regional type, about 2–3 kb in length and contained no consensus or repeat elements. Centromeres in primitive eukaryotic plant cells may have originated from these regional type centromeres.