Determination of Residual Tetracyclines in Meats by High Performance Liquid Chromatography
Determination of Residual Tetracyclines in Meats by High Performance Liquid Chromatography
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高效液相色谱法测定肉类中四环素残留量
DOI：
10.3358/shokueishi.30.42
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
R. Andoh
中科院分区：
文献类型：
--
作者：
Y. Yoneda;Noriko Furushiro;R. Andoh
采用麦氏液(pH 5.5)-甲醇(3：7)提取样品中的土霉素(OTC)、四环素(TC)和金环素(CTC)，建立了镁离子络合高效液相色谱法测定肉类中四环素类药物残留量的方法。提取物经浓缩后用石油醚脱脂。脱脂溶液通过Sep-pak C18色谱柱。用水洗涤色谱柱后，用甲醇洗脱。洗脱液蒸发至干涸。残留物溶于含50 mM醋酸镁-甲醇(77：23)的1M咪唑缓冲液中，进样YMC-PACK-ODS型高效液相色谱柱。以含50 mm醋酸镁-甲醇(77：23)的1M咪唑缓冲液为流动相，荧光检测器检测，添加0.5、2ppm的OTC、TC和2.10ppm的CTC的牛肉中TCS的回收率分别为81.7-82.8%、65.9-80.7%和53.0-80.1%。
A simple and sensitive method for determination of residual tetracyclines (TCs) in meats by high performance liquid chromatography (HPLC) after chelate formation with magnesium ion was developed.Oxytetracycline (OTC), tetracycline (TC) and chlortetracycline (CTC) in a sample were extracted with McIlvaine buffer (pH 5.5)-methanol (3:7). The extract was concentrated and defatted with petroleum ether. The defatted solution was passed through a Sep-pak C18 cartridge. After washing the cartridge with water, TCs were eluted with methanol. The eluate was evaporated to dryness. The residue was dissolved in 1M imidazole buffer containing 50mM magnesium acetate-methanol (77:23), and the solution was injected into a YMC-PACK ODS HPLC column. TCs were separated with 1M imidazole buffer containing 50 mM magnesium acetate-methanol (77:23) as a mobile phase and detected with a flourometric detector.The recoveries of TCs from beef fortified with 0.5, 2ppm of OTC and TC and 2, 10ppm of CTC were 81.7-82.8% for OTC, 65.9-80.7% for TC and 53.0-80.1% for CTC.