Expression of the transcripts of the sigma factors and putative sigma factor regulators of Chlamydia trachomatis L2
Expression of the transcripts of the sigma factors and putative sigma factor regulators of Chlamydia trachomatis L2
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DOI：
10.1016/s0378-1119(00)00094-9
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发表时间：
2000-04-18
期刊：
影响因子：
3.5
通讯作者：
Hatch, TP
中科院分区：
文献类型：
--
作者：
Douglas, AL;Hatch, TP
采用逆转录-聚合酶链式反应（RT-PCR）技术检测了沙眼衣原体L2衣原体RNA聚合酶基因β亚基（rpoC）、3个sigma因子基因（rpoD、ron和rpsD）和4个sigma因子调控基因（rsbW、rsbV1、rsbV2和rsbU）在衣原体发育周期中的稳态水平。rpoC和主要sigma因子rpoD转录本在感染后的任何时候都被检测到，这与它们在管家基因表达中的预期功能一致。替代sigma因子和推定的调控基因的转录本（rsbV2除外，它们在任何时候都以接近恒定的水平存在）在周期早期的初级体（EB）向网状体转化时呈低水平或无法检测到，但在RBs的对数生长阶段很容易检测到。这表明这些基因不是以级联方式表达的，它们的主要作用不太可能是识别特定阶段基因的启动子。(C) 2000 Elsevier Science B.V.版权所有
The steady state levels of the transcripts of the beta' subunit of RNA polymerase gene (rpoC), three sigma factor genes (rpoD, rpoN, and rpsD), and four putative sigma factor regulatory genes (rsbW, rsbV1, rsbV2, and rsbU) of Chlamydia trachomatis L2 were examined during the chlamydial developmental cycle by reverse transcription-polymerase chain reaction (RT-PCR) analysis. rpoC and the major sigma factor rpoD transcripts were detected at all times post-infection, consistent with their expected function in the expression of housekeeping genes. Transcripts of the alternative sigma factors and the putative regulatory genes (with the exception of those of rsbV2, which were present at near constant levels at all times) were present at low or undetectable levels at the time of elementary body (EB) to reticulate body conversion early in the cycle, but were easily detected during the logarithmic growth phase of RBs, indicating that these genes are not expressed in a cascade fashion and that it is unlikely that their major role is to recognize the promoters of stage-specific genes. (C) 2000 Elsevier Science B.V. All rights reserved.