Cg-IκB, a new member of the IκB protein family characterized in the pacific oyster Crassostrea gigas
Cg-IκB, a new member of the IκB protein family characterized in the pacific oyster Crassostrea gigas
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DOI：
10.1016/j.dci.2007.06.001
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发表时间：
2008-01-01
影响因子：
2.9
通讯作者：
Escoubas, J. M.
中科院分区：
文献类型：
--
作者：
Montagnani, C.;Labreuche, Y.;Escoubas, J. M.
NF-kappa B抑制剂（I kappa B）被认为是Rel/NF-kappa B信号通路的主要组成部分，这是动物王国中免疫反应的重要介质。在这里，我们报道了双壳软体动物cDNA的第一个特征，该基因编码一种新的I kappa B成员Cg-I kappa B，显示了大多数I kappa B蛋白特征的保守性。序列和系统发育分析表明，Cg-I kappa B不仅与其他软体动物和昆虫的I kappa B样蛋白具有高度的同一性，而且与脊椎动物的I kappa B- α和-epsilon亚型也具有相似性。表达分析表明，该转录物在牡蛎的所有组织中广泛表达。这项工作与我们之前在长牡蛎中发现的NF-kappa B通路的几个成员一致，并进一步支持了大多数后生动物免疫基因调控保守方案的假设。(c) 2007 Elsevier Ltd.版权所有。
Inhibitors of NF-kappa B (I kappa Bs) have been implicated as major components of the Rel/NF-kappa B signaling pathway, which is an important mediator of immune responses throughout much of the animal kingdom. Here we report the first characterization of a bivalve mollusc cDNA that encodes a novel I kappa B member called Cg-I kappa B showing the conservation of most I kappa B protein characteristics. Sequences as well as phylogenetic analyses reveal a high level of identity between not only Cg-I kappa B and other mollusc and insect I kappa B-like proteins but also similarities to vertebrate I kappa B-alpha and -epsilon isoforms. Expression analyses demonstrated that the transcript is widely expressed in all oyster tissues. This work is consistent with our previous discovery of several members of an NF-kappa B pathway in Crassostrea gigas and further sustains the hypothesis of a conserved scheme of immune gene regulation through most of the metazoans. (c) 2007 Elsevier Ltd. All rights reserved.