Polydopamine-based superparamagnetic molecularly imprinted polymer nanospheres for efficient protein recognition.
Polydopamine-based superparamagnetic molecularly imprinted polymer nanospheres for efficient protein recognition.
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DOI：
10.1016/j.colsurfb.2014.09.018
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发表时间：
2014-11
期刊：
影响因子：
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通讯作者：
Fang Lan;Shaohua Ma;Qi Yang;Liqin Xie;Yao Wu;Z. Gu
中科院分区：
文献类型：
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作者：
Fang Lan;Shaohua Ma;Qi Yang;Liqin Xie;Yao Wu;Z. Gu
本文描述了一种合成超顺磁分子印迹聚合物纳米球 (MIPNS) 的新策略，以实现有效的蛋白质识别。采用改进的细乳液聚合法制备了均质羟基官能化 Fe3O4/聚甲基丙烯酸甲酯 (PMMA) 复合纳米球。在溶菌酶（lyz）模板存在下，通过多巴胺（DA）在 Fe3O4/PMMA 复合纳米球表面的自聚合获得了均匀的超顺磁性 MIPNS。合成的 Fe3O4/PMMA/PDA MIPNS 平均直径为 180 nm、高饱和磁化强度和良好的磁响应。 lyz印迹Fe3O4/PMMA/PDA MIPNS对lyz模板表现出特异性识别和高效吸附能力。吸附在 lyz 印迹 Fe3O4/PMMA/PDA MIPNS 上的 lyz 量比 Fe3O4/PMMA/PDA 非印迹聚合物纳米球（NIPNS）大约 4 倍，分别比 BSA、BHb 和细胞色素 C 约大 14、5 和 5 倍。
A new strategy for synthesis of superparamagnetic molecularly imprinted polymer nanospheres (MIPNSs) for efficient protein recognition is described here. Homogeneous hydroxyl group functionalized Fe3O4/polymethyl methacrylate (PMMA) composite nanospheres were prepared using improved miniemulsion polymerization. Uniform superparamagnetic MIPNSs were obtained via self-polymerization of dopamine (DA) on the surface of Fe3O4/PMMA composite nanospheres in the presence of lysozyme (lyz) template. The as-synthesized Fe3O4/PMMA/PDA MIPNSs had average diameters of 180 nm, high saturation magnetization and a good magnetic response. The lyz-imprinted Fe3O4/PMMA/PDA MIPNSs exhibited specific recognition and efficient adsorption capacity toward lyz template. The amount of lyz adsorbed onto the lyz-imprinted Fe3O4/PMMA/PDA MIPNSs was about 4 times greater than that of the Fe3O4/PMMA/PDA non-imprinted polymer nanospheres (NIPNSs) and about 14, 5, and 5 times greater than that of BSA, BHb, and cyt C, respectively.