Isolation of Biphenyl and Polychlorinated Biphenyl-Degrading Bacteria and Their Degradation Pathway
Isolation of Biphenyl and Polychlorinated Biphenyl-Degrading Bacteria and Their Degradation Pathway
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DOI：
10.1007/s12010-013-0191-5
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发表时间：
2013-05-01
影响因子：
3
通讯作者：
Kikuchi, Shintaro
中科院分区：
文献类型：
--
作者：
Chang, Young-Cheol;Takada, Kazunori;Kikuchi, Shintaro
从某污水中分离到4株联苯降解菌，经16 SrRNA序列分析，分别鉴定为红球菌属（SK-1、SK-3、SK-4）和水生微菌属（SK-2）。其中SK-2菌株对联苯的降解能力最强。当使用0.65，1.3，2.6，或3.9 mM的联苯，联苯被完全降解，分别在24和96小时的培养。然而，在6.5和9.75 mM联苯的情况下，培养120 h后联苯降解产率分别为约80%和46.7%。该菌株在联苯存在下能降解包括高氯多氯联苯（PCB）同系物在内的广谱芳香族化合物。此外，SK-2菌株还能利用含有1 ~ 6个氯取代基的多氯联苯同系物，如2，2 '，4，4'，5，5 '-六氯联苯。SK-2菌株对多氯联苯的利用率高于其他多氯联苯同系物利用菌。4株菌将4-氯联苯代谢为4-氯苯甲酸和2-羟基-6-氧代-6-（4 '-氯苯基）-己-2，4-二烯酸。这些结果表明，分离的菌株可能是良好的候选人，多氯联苯污染的土壤，特别是高盐土壤的生物修复。
Four strains of biphenyl-degrading bacteria were isolated from a sewage and identified from the Rhodococcus genus (SK-1, SK-3, and SK-4) and Aquamicrobium genus (SK-2) by 16S rRNA sequence. Among these strains, strain SK-2 was most suitable for biphenyl degradation. When 0.65, 1.3, 2.6, or 3.9 mM of biphenyl was used, the biphenyl was completely degraded within 24 and 96 h of culture, respectively. However, in the case of 6.5 and 9.75 mM of biphenyl, the biphenyl degradation yields were about 80 % and 46.7 % after 120 h of culture, respectively. The isolated strains could degrade a broad spectrum of aromatic compounds including high-chlorinated polychlorinated biphenyl (PCB) congeners in the presence of biphenyl. In addition, strain SK-2 could utilize PCB congeners containing one to six chlorine substituents such as 2,2',4,4',5,5'-hexachlorobiphenyl. The PCB utilization rate by the strain SK-2 was increased compared to that of other PCB congener-utilizing bacteria. The four isolates metabolized 4-chlorobiphenyl to 4-chlorobenzoic acid and 2-hydroxy-6-oxo-6-(4'-chlorophenyl)-hexa-2,4-dienoic acid. These results suggest the isolated strains might be good candidates for the bioremediation of PCB-contaminated soil, especially high-saline soils.