Immobilization of acetylcholinesterase on one-dimensional gold nanoparticles for detection of organophosphorous insecticides
Immobilization of acetylcholinesterase on one-dimensional gold nanoparticles for detection of organophosphorous insecticides
复制标题
一维金纳米颗粒固定乙酰胆碱酯酶用于检测有机磷杀虫剂
DOI：
10.1007/s11426-010-0105-0
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发表时间：
2010-04-01
影响因子：
9.6
通讯作者：
Han Min
中科院分区：
文献类型：
--
作者：
Li YanRong;Gan ZhiYong;Han Min
报道了一种将乙酰胆碱酯酶(AChE)固定在一维金(Au)纳米粒子上检测有机磷(OP)杀虫剂的方法。用电沉积法制备了一维Au纳米颗粒，然后用2.0M氢氧化钠溶液去除氧化铝模板的气孔。用X射线衍射仪和场发射扫描电子显微镜对其进行了表征。固定化的AChE保持了其生物活性，并催化乙酰硫胆碱的水解生成硫代胆碱，硫代胆碱随后被氧化产生可检测的信号。基于OP杀虫剂对AChE酶活性的抑制作用，对OP杀虫剂甲胺磷进行了灵敏检测。在最佳条件下，该传感器可用于甲胺磷的测定，检测范围为0.004~24mU g/m L，检出限为0.001 m g/m L。所研制的有机磷杀虫剂生物传感器具有良好的稳定性和重复性。本工作表明，一维Au纳米粒子可以作为固定化AChE的理想载体，用于制备相应的生物传感器。
This paper reports a simple method for immobilization of acetylcholinesterase (AChE) on one-dimensional (1D) gold (Au) nanoparticles for detection of organophosphorous (OP) insecticides. 1D Au nanoparticles were prepared by electrodeposition in the pores of an alumina template which was subsequently removed by 2.0 M NaOH solution. They were characterized by XRD and FESEM. The immobilized AChE retained its biological activity and catalyzed the hydrolysis of acetylthiocholine to form thiocholine, which was subsequently oxidized to produce detectable signals. Based on the inhibition toward the enzymatic activity of AChE by OP insecticides, sensitive detection of methamidophos (an OP insecticide) was performed. Under optimal conditions, the sensors could be used for the determination of methamidophos ranging from 0.004 to 24 mu g/mL with the detection limit of 0.001 mu g/mL. The developed OP insecticide biosensors exhibited satisfactory stability and reproducibility. This work demonstrated that 1D Au nanoparticles could serve as an ideal carrier for immobilization of AChE to fabricate the corresponding biosensor.