Characterization of a ubiquitin-fusion gene from the tobacco hawkmoth, Manduca sexta.
Characterization of a ubiquitin-fusion gene from the tobacco hawkmoth, Manduca sexta.
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烟草天蛾 (Manduca sexta) 泛素融合基因的表征。
DOI：
10.1093/nar/18.20.6039
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发表时间：
1990
影响因子：
14.9
通讯作者：
Schwartz,LM
中科院分区：
文献类型：
--
作者：
Bishoff,ST;Schwartz,LM
从天蛾（Manducasexta）的节间肌（ISM）cDNA文库中分离出编码泛素融合蛋白的基因。与来自不同物种的同源蛋白质序列相比，该融合蛋白的预测氨基酸序列高度保守。Manducaclone编码这种泛素融合基因杂交与一个单一的，大量表达的转录本在所有组织检查。在ISM中，转录本以高水平存在，独立于这些肌肉的发育阶段或激素治疗。来自其他物种的数据表明，泛素融合基因参与核糖体的生物合成。
A gene encoding a ubiquitin-fusion protein was isolated from a cDNA library made from the intersegmental muscles (ISM) of the moth,Manduca sexta. The predicted amino acid sequence of this fusion protein is highly conserved when compared to the sequence of homologous proteins from diverse species. TheManducaclone encoding this ubiquitin fusion gene hybridized with a single, abundantly expressed transcript in all tissues examined. In the ISM, the transcript was present at high levels, independent of the developmental stage or hormonal treatment of these muscles. Data from other species indicate that ubiquitin-fusion genes participate in ribosome biogenesis.