The developmental cycle of Ehrlichia chaffeensis in vertebrate cells
The developmental cycle of Ehrlichia chaffeensis in vertebrate cells
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DOI：
10.1111/j.1462-5822.2006.00812.x
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发表时间：
2007-03-01
影响因子：
3.4
通讯作者：
Yu, Xue-jie
中科院分区：
文献类型：
--
作者：
Zhang, Jian-zhi;Popov, Vsevolod L.;Yu, Xue-jie
查菲埃里希体是一种专性细胞内细菌，在哺乳动物细胞中有两种形式：具有致密类核的小致密核心细胞（DC）和具有均匀分散的类核的较大网状细胞（RC）。我们已经确定，通过电子显微镜，DC，而不是RC附着并进入宿主细胞和RC，而不是DC在宿主细胞内的倍数。共聚焦显微镜分析外膜蛋白表达结果表明，RC表达28kDa的外膜蛋白（p28），RC向DC转化的中间体表达gp120和p28，成熟DC仅表达gp120。DC的TCID50比RC高6 log（10）。我们的结论是E. DC附着并进入宿主细胞，转化为RC，RC经48 h二分裂增殖，72 h成熟为DC。
Ehrlichia chaffeensis, an obligatory intracellular bacterium, has two forms in mammalian cells: small dense-cored cells (DC) with dense nucleoid and larger reticulate cells (RC) with uniformly dispersed nucleoid. We have determined by electron microscopy that DC but not RC attaches to and enters into the host cells and RC but not DC multiples inside the host cells. Analysis of outer membrane protein expression by confocal microscopy showed that RC expressed the 28 kDa outer membrane protein (p28), the intermediate form, which were transforming from RC to DC, expressed both gp120 and p28, and the mature DC expressed gp120 only. The TCID50 of DC is 6 log(10) higher than RC. We conclude that E. chaffeensis has a developmental cycle, in which the DC attaches to and enters into the host cells, and transforms into RC and the RC multiplies by binary fission for 48 h and then matures into DC at 72 h.