Expression of gastric-associated antigens by human premalignant and malignant colonic epithelial cells.
Expression of gastric-associated antigens by human premalignant and malignant colonic epithelial cells.
复制标题
人类癌前和恶性结肠上皮细胞表达胃相关抗原。
作者：
Higgins,PJ;Friedman,E;Lipkin,M;Hertz,R;Attiyeh,F;Stonehill,EH
制备第2个三个月的胎儿器官提取物和吸收成人组织（抗STFa）的兔抗血清用于检测常见的抗原第2个三个三个月的胎儿大肠，正常成人胃，几个结肠癌细胞（在原代和建立的文化）和上皮细胞培养的良性结肠息肉。抗STFa阳性细胞的频率是最高的文化来源于良性肿瘤已知与更大程度的癌前病变。因此，抗原阳性细胞的百分比从18%和43%增加到70%的培养物从管状，绒毛管状和绒毛状腺瘤，分别。在阳性培养物的任何给定区域内，含抗原细胞的分布明显存在相当大的异质性。吸收实验，使用胎儿和正常成人组织提取物的光谱，表明腺瘤癌特异性存在于胎儿，但不是成人，大肠和正常成人胃。
A rabbit antiserum prepared to 2nd-trimester fetal organ extracts and absorbed with adult tissue (anti-STFa) was used to detect antigens common to 2nd-trimester fetal large bowel, normal adult stomach, several colon carcinoma cells (in primary and established cultures) and epithelial cells cultured from benign colonic polyps. The frequency of anti-STFa-positive cells was highest in cultures derived from benign tumors known to be associated with a greater degree of premalignancy. Thus, the percentage of antigen-positive cells increased from 18 and 43% to 70% of cultures from tubular, villotubular and villous adenomas, respectively. Considerable heterogeneity in the distribution of antigen-containing cells was evident within any given area of a positive culture. Absorption experiments, using a spectrum of fetal and normal adult tissue extracts, indicated that the adenoma-carcinoma specificity resides in fetal, but not adult, large bowel and normal adult stomach.