Erythrocyte structure and dynamics quantified by Hilbert phase microscopy
Erythrocyte structure and dynamics quantified by Hilbert phase microscopy
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DOI：
10.1117/1.2149847
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发表时间：
2005-11-01
影响因子：
3.5
通讯作者：
Feld, MS
中科院分区：
文献类型：
--
作者：
Popescu, G;Ikeda, T;Feld, MS
我们提出了一种新的定量研究红细胞形态和动力学的方法。该仪器集成了定量相位显微镜和倒置显微镜，这使得它特别适合于活红细胞的非侵入性评估。特别是，我们证明了这种方法的能力，以量化非侵入性的细胞体积和动态形态。在毫秒时间尺度下的亚纳米路径长度灵敏度通过测量溶血期间从细胞流出的血红蛋白来举例说明。(C)2005年，由光学仪器工程师学会（Society of Photo-Optical Instrumentation Engineers）主办。
We present a new quantitative method for investigating red blood cell morphology and dynamics. The instrument integrates quantitative phase microscopy with an inverted microscope, which makes it particularly suitable for the noninvasive assessment of live erythrocytes. In particular, we demonstrate the ability of this approach to quantify noninvasively cell volume and dynamic morphology. The subnanometer path-length sensitivity at the millisecond time scales is exemplified by measuring the hemoglobin flow out of the cell during hemolysis. (C) 2005 Society of Photo-Optical Instrumentation Engineers.