ISOLATION AND ANALYSIS OF COMPLEMENT ACTIVATING AGGREGATES FROM SYNOVIAL-FLUID OF PATIENTS WITH RHEUMATOID-ARTHRITIS USING MONOCLONAL ANTI-C3D ANTIBODIES
ISOLATION AND ANALYSIS OF COMPLEMENT ACTIVATING AGGREGATES FROM SYNOVIAL-FLUID OF PATIENTS WITH RHEUMATOID-ARTHRITIS USING MONOCLONAL ANTI-C3D ANTIBODIES
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DOI：
10.1136/ard.46.1.55
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发表时间：
1987-01-01
影响因子：
27.4
通讯作者：
CZUDEK, R
中科院分区：
文献类型：
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作者：
BEDWELL, AE;ELSON, CJ;CZUDEK, R
用固相结合的抗C3d单抗分离类风湿关节炎(RA)患者滑液中的补体激活聚集体，并对结合物质的含量进行了分析。在RA滑液中发现高水平的C3d聚集的免疫球蛋白，且抗C3d琼脂糖凝胶结合的主要免疫球蛋白为Ig G。根据C3d水平判断，携带C3d的聚集免疫球蛋白水平与补体激活之间显示出很强的相关性。C3d水平与补体消耗和C1q结合活性之间也观察到显著(但不那么强烈)的关系。C3d水平和携带C3d的聚集免疫球蛋白水平均与RA滑液中发现的多形核白细胞(PMN)数量显著相关。这些结果表明，从RA滑液中分离出的携带C3d的聚集免疫球蛋白(尤其是Ig G)负责激活补体并吸引PMN进入关节间隙。放射免疫分析显示，抗C3d抗体结合的类风湿因子(RF)活性与C3d聚集的Ig G(或Ig M)水平之间无相关性。此外，大多数滑液聚乙二醇沉淀物中的抗C3d琼脂糖凝胶结合的物质既不含IgM，也不含IgGRF。因此，这两种技术都表明，大多数含C3d的络合物不含RF。由于补体固定聚集体显然只包含免疫球蛋白和补体成分，结果提出了聚集体是如何形成的问题。这表明，在抗原或抗体(RF)从复合体中解离后，RA-Ig G可能保持聚集状态。
The complement activating aggregates in synovial fluids of patients with rheumatoid arthritis (RA) have been isolated using monoclonal IgM anti-C3d antibodies attached to solid phases, and the content of the material bound has been analysed. High levels of aggregated IgG bearing C3d were found in RA synovial fluids, and IgG was the major immunoglobulin bound from such synovial fluids by anti-C3d Sepharose. A strong correlation was shown between levels of aggregated IgG bearing C3d and complement activation, as judged by C3d levels. Significant (but less strong) relationships were also observed between C3d levels and both complement consuming and C1q binding activity. C3d levels and levels of aggregated IgG bearing C3d were both significantly associated with the numbers of polymorphonuclear leucocytes (PMNs) found in RA synovial fluids. From these results it is concluded that the aggregated immunoglobulins bearing C3d (particularly IgG) isolated from RA synovial fluids are responsible for activating complement and attracting PMNs into the joint space. Radioimmunoassay showed no correlation, however, between levels of aggregated IgG (or IgM) bearing C3d and rheumatoid factor (RF) activity bound by anti-C3d. In addition, the material bound by anti-C3d Sepharose from most synovial fluid polyethylene glycol precipitates did not contain either IgM or IgG RF. Thus both techniques show that the majority of complexes bearing C3d do not contain RF. As the complement fixing aggregates apparently contain only immunoglobulin and complement components of the results raise the problem of how the aggregates are formed. It is suggested that RA IgG may remain aggregated after either antigen or antibody (RF) has dissociated from the complex.