Heat stress increases myonuclear number and fiber size via satellite cell activation in rat regenerating soleus fibers
Heat stress increases myonuclear number and fiber size via satellite cell activation in rat regenerating soleus fibers
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DOI：
10.1152/japplphysiol.91651.2008
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发表时间：
2009-11-01
影响因子：
3.3
通讯作者：
Ohira, Yoshinobu
中科院分区：
文献类型：
--
作者：
Oishi, Yasuharu;Hayashida, Mari;Ohira, Yoshinobu
Oishi Y，Hayashida M，Tsukiashi S，Taniguchi K，甲米K，Roy RR，Ohira Y.热应激通过激活大鼠比目鱼肌再生纤维中的卫星细胞增加肌纤维的数量和尺寸。J Appl Physiol 107：1612-1621，2009.首次发表于2009年6月25日; doi：10.1152/japplphysiol.91651.2008。为了研究热应激（高温）对肌肉变性再生的影响，成年雄性Wistar大鼠比目鱼肌双侧注射布比卡因。将大鼠分配到久坐对照组（Con）、热应激组（Heat）、布比卡因注射组（BPVC）或布比卡因注射加热应激组（BPVC + Heat）。在热和BPVC +热组中通过将下半身浸入保持在42 +/- 1摄氏度的水中30分钟48小时后注射布比卡因和在接下来的1或2周期间每隔一天诱导热应激。最后一次热应激后24 h，切除各组比目鱼肌。在两个时间点，BPVC组的平均肌肉重量、纤维横截面积、肌纤维数量、热休克蛋白72（Hsp 72）和钙调神经磷酸酶蛋白水平均低于Con组或Heat组。相比之下，在1周和/或2周时间点，BPVC +加热组中的这些参数中有几个与Con或加热组没有差异或更高。通过分析Pax 7阴性、M-钙粘蛋白阴性和MyoD阳性细胞核估计的总卫星细胞和活化卫星细胞数量，BPVC + Heat组高于所有其他组。结合，结果表明，热应激相关的卫星细胞的激活和热休克蛋白72和钙调神经磷酸酶的表达上调在布比卡因注射后比目鱼肌纤维的再生中发挥了重要作用。
Oishi Y, Hayashida M, Tsukiashi S, Taniguchi K, Kami K, Roy RR, Ohira Y. Heat stress increases myonuclear number and fiber size via satellite cell activation in rat regenerating soleus fibers. J Appl Physiol 107: 1612-1621, 2009. First published June 25, 2009; doi:10.1152/japplphysiol.91651.2008.-To investigate the effects of heat stress (hyperthermia) on muscle degeneration-regeneration, the soleus muscles of adult male Wistar rats were injected bilaterally with a single injection of bupivacaine. The rats were assigned to a sedentary control (Con), heat stress (Heat), bupivacaine-injected (BPVC), or bupivacaine-injected plus heat stress (BPVC + Heat) group. Heat stress was induced in the Heat and BPVC + Heat groups by immersion of the lower half of the body into water maintained at 42 +/- 1 degrees C for 30 min 48 h after the injection of bupivacaine and every other day during the following 1 or 2 wk. The soleus muscles in all groups were excised 24 h after the final bout of heat stress. Mean muscle weight, fiber cross-sectional area, myonuclear number, and heat shock protein 72 (Hsp72) and calcineurin protein levels were lower in the BPVC than in the Con or Heat groups at both time points. In contrast, several of these parameters in the BPVC + Heat group were not different or higher than in the Con or Heat groups at the 1- and/or 2-wk time points. The number of total and activated satellite cells, estimated by analyses of Pax7-negative, M-cadherin-negative, and MyoD-positive nuclei, was greater in BPVC + Heat than in all other groups. Combined, the results indicate that heat stress-related activation of satellite cells and upregulation of Hsp72 and calcineurin expression played important roles in the regeneration of the soleus fibers after bupivacaine injection.