Regulation of the Friction Coefficient of Articular Cartilage by TGF-β1 and IL-1β
Regulation of the Friction Coefficient of Articular Cartilage by TGF-β1 and IL-1β
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DOI：
10.1002/jor.20713
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发表时间：
2009-02-01
影响因子：
2.8
通讯作者：
Reddi, A. Hari
中科院分区：
文献类型：
--
作者：
DuRaine, Grayson;Neu, Corey P.;Reddi, A. Hari
关节软骨的作用是为关节运动提供低摩擦表面，使其在数十年的寿命中都可以使用。浅层区蛋白（SZP）是关节软骨浅层软骨细胞分泌的一种糖蛋白，有助于有效的边界润滑。在细胞和外植体培养物中，TGF-β1 和 IL-1β 已被证明分别上调和下调 SZP 蛋白水平。据推测，这些细胞因子也可以通过 SZP 调节来调节关节软骨的摩擦系数。使用钉盘式摩擦计测量由于在边界润滑状态下滑动而导致的软骨外植体（未经处理和经TGF-1β或IL-1β处理）与光滑玻璃表面之间的摩擦系数。使用酶联免疫吸附测定对 SZP 进行定量，并通过免疫组织化学进行定位。 TGF-β1和IL-1β治疗均导致关节软骨的摩擦系数以位置和时间依赖性方式降低。 TGF-β 1 处理引起的摩擦系数变化与浅层区域内 SZP 染色深度的增加相对应，而 IL-1 β 处理引起的摩擦系数变化与 SZP 染色深度无关。然而，IL-1β处理引起的变化与表面粗糙度的变化相对应，这是通过对原子力显微镜获得的表面图像的分析确定的。这些发现表明，关节软骨的低摩擦可以通过 TGF-β1 和 IL-1β 治疗来改变，并且摩擦系数取决于多种因素，包括 SZP 定位和表面粗糙度。 2008年骨科研究会。由 Wiley periodicals, Inc. 出版。J Orthop Res 27:249-256。 2009年
Articular cartilage functions to provide a low-friction surface for joint movement for many decades of life. Superficial zone protein (SZP) is a glycoprotein secreted by chondrocytes in the superficial layer of articular cartilage that contributes to effective boundary lubrication. In both cell and explant cultures, TGF-beta 1 and IL-1 beta have been demonstrated to, respectively, upregulate and downregulate SZP protein levels. It was hypothesized that the friction coefficient of articular cartilage could also be modulated by these cytokines through SZP regulation. The friction coefficient between cartilage explants (both untreated and treated with TGF-1 beta or IL-1 beta) and a smooth glass surface due to sliding in the boundary lubrication regime was measured with a pin-on-disk tribometer. SZP was quantified using an enzyme-linked immunosorbant assay and localized by immunohistochemistry. Both TGF-beta 1 and IL-1 beta treatments resulted in the decrease of the friction coefficient of articular cartilage in a location- and time-dependent manner. Changes in the friction coefficient clue to the TGF-beta 1 treatment corresponded to increased depth of SZP staining within the superficial zone, while friction coefficient changes due to the IL-1 beta treatment were independent of SZP depth of staining. However, the changes induced by the IL-1 beta treatment corresponded to changes in surface roughness, determined from the analysis of surface images obtained with an atomic force microscope. These findings demonstrate that the low friction of articular cartilage can be modified by TGF-beta 1 and IL-1 beta treatment and that the friction coefficient depends on multiple factors, including SZP localization and surface roughness. 2008 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 27:249-256. 2009