Reinforcement of transvaginal repair using polypropylene mesh functionalized with basic fibroblast growth factor.
Reinforcement of transvaginal repair using polypropylene mesh functionalized with basic fibroblast growth factor.
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DOI：
10.1016/j.colsurfb.2016.02.034
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发表时间：
2016-06
期刊：
影响因子：
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通讯作者：
Dandan Zhang;Z. Lin;Ruoyu Cheng;Wei Wu;Jia Yu;Xin Zhao;Xinliang Chen;W. Cui
中科院分区：
文献类型：
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作者：
Dandan Zhang;Z. Lin;Ruoyu Cheng;Wei Wu;Jia Yu;Xin Zhao;Xinliang Chen;W. Cui
在过去的二十年中，已经开发了许多改进，旨在通过使用聚丙烯（PP）补片植入物改善盆腔器官脱垂（POP）的经阴道修复。然而，PP的疏水性对将潜在技术转化为可行的临床应用提出了很大的障碍。在这项研究中，通过操纵多巴胺的自聚合和强粘附特性，我们开发了一种简便的方法，通过用聚多巴胺（PDA）修饰PP网片来增强经阴道修复，该方法允许将碱性成纤维细胞生长因子（bFGF）容易地接枝到PP表面。发现用bFGF对PP网片的这种表面改性有效地促进生物活性，而不改变PP网片的形态或机械性能。此外，与未改性的PP相比，bFGF改性的PP网片显著促进了细胞活力和粘附。最终，在植入三个月后，bFGF改性PP补片表现出改善的组织修复，沉积胶原的组织化程度更高，抗张强度增加，炎症反应减少。总体而言，表面改性PP补片作为POP治疗中经阴道修补术的模板具有高度实用性。
Numerous modifications have been developed over the past two decades seeking to improve the transvaginal repair in the pelvic organ prolapse (POP) by using polypropylene (PP) mesh implants. The hydrophobicity of PP, however, presents a great hindrance for translating potential technologies into viable clinical applications. In this study, by manipulating self-polymerization and strong adhesive characteristics of dopamine, we developed a facile method to enhance the transvaginal repair by modifying PP meshes with polydopamine (PDA), which allowed easy grafting of basic fibroblast growth factor (bFGF) onto the surface of PP. Such surface modification of PP meshes with bFGF was found to efficiently promote bioactivity without changing the morphology or mechanical properties of the PP meshes. Additionally, bFGF-modified PP meshes significantly promoted cell viability and adhesion compared to the unmodified PP. Ultimately, after three months of implantation, the bFGF-modified PP meshes exhibited improved tissue repair with greater degree of organization of deposited collagen, increased tensile strength and reduced inflammatory response. Overall, the surface-modified PP meshes will be highly practical as templates for transvaginal repair in the POP treatment.