Succession and diversity of microorganisms and their association with physicochemical properties during green waste thermophilic composting
Succession and diversity of microorganisms and their association with physicochemical properties during green waste thermophilic composting
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DOI：
10.1016/j.wasman.2017.12.026
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发表时间：
2018-03-01
期刊：
影响因子：
8.1
通讯作者：
Zhang, Bolin
中科院分区：
文献类型：
--
作者：
Liu, Ling;Wang, Shuqi;Zhang, Bolin
实现了与绿色废物堆肥高温阶段相关的细菌多样性的全面表征。在本研究中，进行了八种不同的处理（T1-T8）和三种重复的实验室规模绿色废物堆肥，以比较纤维素酶（即0、2％）、微生物接种量（即0、2和4％）和颗粒尺寸（即2和5μmm）对细菌群落结构的影响。观察T1-T8堆肥的理化特性和细菌群落，并通过MiSeq平台进行高通量测序检查细菌结构和多样性。结果表明，各处理中最丰富的门是厚壁菌门、绿屈菌门和变形菌门。香农指数和非度量多维标度（NMDS）显示堆肥中期细菌丰度和多样性较高。与5毫米处理相比，2毫米粒径的细菌群落多样性更加丰富。添加纤维素酶和微生物菌剂可以提高发酵温度，降低堆肥pH和C/N比，从而获得更高的GI指数。 2mm粒径处理的腐殖质（HS）和腐植酸（HA）含量高于5mm粒径处理。典型对应分析表明，细菌丰度和多样性的差异与HA、E4/E6和温度显着相关，并且细菌多样性与环境参数的关系受堆肥阶段的影响。基于这些结果，应用纤维素酶促进绿色废物堆肥是可行的，并且粒径被确定为堆肥理化特性和细菌多样性的潜在控制因素。 (C) 2017 Elsevier Ltd. 保留所有权利。
A comprehensive characterization of the bacterial diversity associated to thermophilic stages of green waste composting was achieved. In this study, eight different treatments (T1-T8) and three replicated lab-scale green waste composting were carried out to compare the effect of the cellulase (i.e. 0, 2%), microbial inoculum (i.e. 0, 2 and 4%) and particle size (i.e. 2 and 5?mm) on bacterial community structure. Physicochemical properties and bacterial communities of T1-T8 composts were observed, and the bacterial structure and diversity were examined by high-throughput sequencing via a MiSeq platform. The results showed that the most abundant phyla among the treatments were the Firmicutes, Chloroflexi and Proteobacteria. The shannon index and non-metric multidimensional scaling (NMDS) showed higher bacterial abundance and diversity at the metaphase of composting. Comparing with 5-mm treatments, particle size of 2-mm had a richer diversity of bacterial communities. The addition of cellulase and a microbial inoculum could promote the fermentation temperature, reduce the compost pH and C/N ratio and result in higher GI index. The humic substance (HS) and humic acid (HA) contents for 2-mm particle size treatments were higher than those of 5-mm treatments. Canonical correspondence analysis suggested that differences in bacterial abundance and diversity significantly correlated with HA, E4/E6 and temperature, and the relationship between bacterial diversity and environmental parameters was affected by composting stages. Based on these results, the application of cellulase to promote green waste composting was feasible, and particle size was identified as a potential control of composting physicochemical properties and bacterial diversity. (C) 2017 Elsevier Ltd. All rights reserved.