Zebrafish protocadherin 10 is involved in paraxial mesoderm development and somitogenesis
Zebrafish protocadherin 10 is involved in paraxial mesoderm development and somitogenesis
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DOI：
10.1002/dvdy.20622
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发表时间：
2006-02-01
影响因子：
2.5
通讯作者：
Yorifuji, H
中科院分区：
文献类型：
--
作者：
Murakami, T;Hijikata, T;Yorifuji, H
本文首次报道了斑马鱼原钙粘蛋白10（Protocadherin 10，Pcdh 10，OL-protocadherin）的分子克隆及其在节板发育中的功能分析。在胚胎中表达的表位标记的Pcdh 10定位于相邻细胞的细胞周边。原位杂交显示，pcdh 10在近轴中胚层（PAM）和发育中的体节、松果体、间脑和耳囊附近表达。PAM的表达在最后几个假定的体节中增加，在最后分割的体节中达到最高水平，此后在体节成熟过程中降低。这些表达模式表明Pcdh 10参与PAM和体节的发育。这一点得到了证实，吗啉敲低和显性负抑制Pcdh 10在胚胎中，扰乱PAM细胞和体节分割的运动。比较研究表明，pcdh 10的表达持续约3倍以上的近轴原钙粘蛋白（pcdh 8）在成熟体节。近轴细胞表达pcdh 8而不表达pcdh 10。我们建议，Pcdh 10参与PAM细胞和体节分割的形态发生运动，Pcdh 8和Pcdh 10的差异粘附在体节和近轴细胞的形态发生机制中发挥作用。
Here, we present the first report of the molecular cloning of zebrafish protocadherin 10 (Pcdh10, OL-protocadherin) and describe its functional analyses in the development of segmental plate. Epitope-tagged Pcdh10 expressed in embryos was localized on cell peripheries of adjacent cells. In situ hybridization showed that pcdh10 was expressed in the paraxial mesoderm (PAM) and developing somites, and in the pineal body, the diencephalon, and the vicinity of otocysts. Expression in PAM increased in the last few presumptive somites, reached the maximum level in the latest segmenting somites, and decreased thereafter during somite maturation. These expression patterns suggested that Pcdh10 is involved in development of PAM and somites. This was confirmed by morpholino knockdown and dominant-negative inhibition of Pcdh10 in embryos, which disturbed movements of PAM cells and somite segmentation. Comparative studies showed that pcdh10 expression lasted up to approximately three times longer in maturing somites than that of paraxial protocadherin (pcdh8). They also indicated that the adaxial cells expressed pcdh8 but not pcdh10. We propose that Pcdh10 is involved in the morphogenic movements of PAM cells and somite segmentation and that differential adhesion of Pcdh8 and Pcdh10 plays a role in the morphogenic machinery of somites and adaxial cells.