Changes in pain threshold and lumbar spinal cord immediate-early gene expression induced by paced and nonpaced mating in female rats.
Changes in pain threshold and lumbar spinal cord immediate-early gene expression induced by paced and nonpaced mating in female rats.
复制标题
雌性大鼠有节奏和无节奏交配引起的疼痛阈值和腰脊髓立即早期基因表达的变化。
DOI：
10.1016/s0006-8993(00)01957-0
复制
发表时间：
2000
期刊：
影响因子：
2.9
通讯作者：
Erskine,MS
中科院分区：
文献类型：
--
作者：
Lee,JW;Erskine,MS
在交配过程中接受阴道颈刺激(VCS)可以诱导疼痛，并抑制腰髓中的FOS免疫反应(FOS-IR)。然而，目前尚不清楚这种对FOS-IR的抑制是否反映了对传入伤害性输入的抑制。本研究通过比较接受交配刺激的发情雌性动物的两种即刻早期基因(IEG)产物FOS和Egr-1在大脑中诱导不同数量的FOS-IR，来检验这两种反应是否与伤害性感受有关。去卵巢的类固醇治疗的大鼠在与雄性有节奏或不有节奏的性接触的条件下交配，直到接受5到15次插入。控制组只接受雄性的坐骑，或者留在自己的家里。在实验I中，与非起搏和仅骑行刺激相比，有节奏的交配导致腰椎6(L6)脊椎节段FOS-IR的显著抑制。这种减少尤其发生在接受五次入院治疗的有节奏的女性中。相比之下，有节奏的雌性在15次入院时的海拔显著高于回家水平，所有没有节奏的雌性，只骑动物。在所有接触男性的女性中，Egr-1免疫反应(Egr-1-IR)细胞的数量均高于平均水平。在实验2中，5次插入(有节奏或无节奏)的雌性在交配后5个S(时间0)内甩尾潜伏期显著增加，而15次插入的雌性在交配后90天内表现出痛觉过敏(15个非起搏)或无变化(15个起搏)。在接受两次射精后立即接受测试的其他女性显示出止痛作用。起搏交配虽然在抑制FOS-IR方面比非起搏交配更有效，但不影响VCS诱导的镇痛的出现。我们得出结论，起搏交配对FOS-IR的抑制与交配诱导的镇痛无关。
Vaginocervical stimulation (VCS) received during mating is known to induce analgesia and to suppress FOS-immunoreactivity (FOS-IR) in lumbar spinal cord. However, it is not known whether this suppression of FOS-IR reflects inhibition of afferent nociceptive input. The present studies examined whether two immediate-early gene (IEG) products, FOS and Egr-1, covary with nociception by comparing both responses in estrous females that received mating stimulation known to induce varying amounts of FOS-IR in brain. Ovariectomized steroid-treated rats were mated under conditions in which they paced or did not pace sexual contacts with males until receiving 5 or 15 intromissions. Control groups received mounts-without-intromission only from males or remained in their homecages. In experiment I, paced mating resulted in a significant overall suppression of FOS-IR in the lumbar 6 (L6) spinal segment compared to nonpaced and mounts only stimulation. This reduction occurred specifically among paced females receiving five intromissions. In contrast, significant elevations above homecage levels were seen in paced females given 15 intromissions, all nonpaced females, and mounts only animals. The numbers of Egr-1-immunoreactive (Egr-1-IR) cells increased equally above homecage levels in all male-exposed females. In experiment II, females that received five intromissions (paced or nonpaced) showed significant increases in tail-flick latency (TFL) within 5 s (time 0) after mating, while females receiving 15 intromissions showed hyperalgesia (15 nonpaced) or no change (15 paced) in TFL throughout 90 s postmating. Additional females tested immediately after receiving two ejaculations showed analgesia. Paced mating, though more effective than nonpaced mating in suppressing FOS-IR, did not influence the appearance of VCS-induced analgesia. We conclude that the suppression of FOS-IR by paced mating is not related to mating-induced analgesia.