CD90 and CD110 correlate with cancer stem cell potentials in human T-acute lymphoblastic leukemia cells
CD90 and CD110 correlate with cancer stem cell potentials in human T-acute lymphoblastic leukemia cells
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DOI：
10.1016/j.bbrc.2009.03.127
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发表时间：
2009-05-29
影响因子：
3.1
通讯作者：
Morimoto, Chikao
中科院分区：
文献类型：
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作者：
Yamazaki, Hiroto;Nishida, Hiroko;Morimoto, Chikao
虽然最近在髓性白血病中发现了肿瘤干细胞（CSCs），但发表的关于淋巴细胞恶性肿瘤的数据却很少。t -急性淋巴细胞白血病（T-ALL）的特点是t细胞前体异常增殖，通常具有侵袭性。由于CD34是T-ALL中CSCs的唯一阳性选择标记，我们对T-ALL细胞系中的CD标记进行了广泛的分析。我们发现一些被测试的细胞系由具有不同水平表面标记表达的异质细胞群组成。特别是，在体外和移植实验中，CD90 （Thy-1）和CD110 （c-Mpl）的一个小亚群被证明与干细胞特性相关。由于这些标记物在造血干细胞上表达，我们的研究结果表明，干细胞样群体在CD90+/CD110+部分中富集，它们是在某些T-ALL病例中分离CSCs的有用的阳性选择标记物。(C) 2009爱思唯尔公司版权所有。
Although cancer stem cells (CSCs) have been recently identified in myeloid leukemia, published data on lymphoid malignancy have been sparse. T-acute lymphoblastic leukemia (T-ALL) is characterized by the abnormal proliferation of T-cell precursors and is generally aggressive. As CD34 is the only positive-selection marker for CSCs in T-ALL, we performed extensive analysis of CD markers in T-ALL cell lines. We found that some of the tested lines consisted of heterogeneous populations of cells with various levels of surface marker expression. In particular, a small subpopulation of CD90 (Thy-1) and CD110 (c-Mpl) were shown to correlate with stem cell properties both in vitro and in transplantation experiments. As these markers are expressed on hematopoietic stem cells, our results suggest that stem cell-like population are enriched in CD90+/CD110+ fraction and they are useful positive-selection markers for the isolation of CSCs in some cases of T-ALL. (C) 2009 Elsevier Inc. All rights reserved.