Apoptotic and proliferative index after alpha-1-adrenoceptor antagonist and/or finasteride treatment in benign prostatic hyperplasia
Apoptotic and proliferative index after alpha-1-adrenoceptor antagonist and/or finasteride treatment in benign prostatic hyperplasia
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DOI：
10.1159/000066120
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发表时间：
2002-01-01
影响因子：
1.6
通讯作者：
Baykara, M
中科院分区：
文献类型：
--
作者：
Erdogru, T;Ciftcioglu, MA;Baykara, M
简介：近年来，诱导细胞凋亡已成为优化良性前列腺增生（BPH）药物治疗的潜在靶点。本实验观察了α 1-肾上腺素能受体拮抗剂（α 1-ARA）、5-α还原酶抑制剂及其联合应用对良性增生前列腺细胞凋亡和增殖指数的影响。材料和方法：共49例男性BPH患者（平均年龄：66.5岁）接受α 1-ARA和/或非那肽治疗，进行了回顾性评价。接受α 1-ARA（多沙唑嗪n=12和特拉唑嗪n=10）、非那肽（n=9）和非那肽与α 1-ARA联合治疗（n=9）的患者入组本研究。一抗为Ki-67和增殖细胞核抗原，用于评价前列腺基质细胞和上皮细胞的增殖。TUNEL法检测细胞凋亡DNA片段化。结果：各治疗组前列腺间质和上皮细胞增殖率无明显变化。非那肽与α 1-ARA、α 1-ARA与非那肽+ α 1-ARA和非那肽+ α 1-ARA与非那肽组的上皮细胞凋亡指数（AI）无统计学显著性。虽然α 1-ARA对间质细胞凋亡的作用比非那司得更有效，但α 1-ARA诱导的间质细胞凋亡与α 1-ARA+非那司得治疗无显著差异。结论：不仅雄激素的变异性，但也改变交感神经传递与年龄可能有重要意义的病理生理前列腺生长。非那肽和α 1-ARA的组合并不上级于α 1-ARA治疗，其具有相似的上皮和基质凋亡作用，细胞增殖不受影响。版权所有（C）2002 S. Karger AG，巴塞尔。
Introduction: The induction of apoptosis has emerged as a potential target for optimization of the medical management of benign prostatic hyperplasia (BPH), recently. The influence of alpha1-adrenoceptor antagonist (alpha1-ARA), 5-alpha reductase inhibitor and their combination on prostatic cell apoptotic and proliferative indices of benign hyperplastic prostate gland were investigated. Material and Methods: A total of 49 male patients with BPH (mean age: 66.5 years) treated with alpha1-ARA and/or finasteride were retrospectively evaluated. Patients treated with alpha1-ARA (doxazosin n=12 and terazosin n=10), finasteride (n=9) and combination of finasteride and alpha1-ARA (n=9) were enrolled in the study. Primary antibodies were Ki-67 and proliferating cell nuclear antigen for the evaluation of proliferation in prostate stromal and epithelial cells. In situ apoptotic DNA fragmentation was evaluated using TUNEL assay. Results: All treatment groups had no significant changes in the rate of prostate stromal and epithelial cell proliferation. Epithelial apoptotic index (AI) was not statistically significant for finasteride vs. alpha1-ARA, alpha1-ARA vs. finasteride + alpha1-ARA and finasteride + alpha1-ARA vs. finasteride groups. While alpha1-ARA was more effective than finasteride on stromal apoptosis, alpha1-ARA-induced stromal apoptosis was not significantly different from alpha1-ARA plus finasteirde treatment. Conclusion: Not only androgen variabilities but also alterations in sympathetic neurotransmission with age could have important implications for pathophysiological prostate growth. The combination of finasteride and alpha1-ARA is not superior to alpha1-ARA therapy with their similar epithelial and stromal apoptotic effects with unaffected cell proliferation. Copyright (C) 2002 S. Karger AG, Basel.