Identification of novel mobile colistin resistance gene mcr-10
Identification of novel mobile colistin resistance gene mcr-10
复制标题
新型移动粘菌素抗性基因mcr-10的鉴定
DOI：
10.1080/22221751.2020.1732231
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发表时间：
2020-01-01
影响因子：
13.2
通讯作者：
Zong, Zhiyong
中科院分区：
文献类型：
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作者：
Wang, Chengcheng;Feng, Yu;Zong, Zhiyong
摘要移动粘菌素耐药(Mcr)基因是一个新的挑战。在这里，我们描述了一个新的mcr基因，mcr-10，位于罗根坎皮肠杆菌临床菌株的IncFIA质粒上。MCR-10与MCR-9的核苷酸同源性最高(79.69%)，编码的氨基酸序列与MCR-9的同源性为82.93%。将mcr-10克隆到对粘菌素敏感的罗根坎皮埃希菌株中，可使粘菌素最低抑菌浓度增加4倍(从1 mg/L增加到4 mgMIC)。通过对GenBank的筛选，在四大洲国家的各种肠杆菌科物种中都发现了mcr-10基因，表明该基因已经广泛传播。MCR-10具有79.04%~83.67%的氨基酸同源性和高度保守的预测蛋白质结构，与不同种丁藻的染色体编码的MCR样磷乙醇胺转移酶(这里称为MCR-B)具有高度保守性。因此，MCR-10、MCR-9和MCR-B蛋白可能起源于共同的祖先。MCR-10与位点特异性重组酶编码基因相邻，被IS903包围，可通过位点特异性重组或复合转座子进行动员。我们的结果表明，mcr-10是一个新的质粒型粘菌素抗性基因，值得立即监测和进一步研究。
ABSTRACT Mobile colistin resistance (mcr) genes represent an emerging challenge. Here we describe a novel mcr gene, mcr-10, on an IncFIA plasmid of an Enterobacter roggenkampii clinical strain. mcr-10 has the highest nucleotide identity (79.69%) with mcr-9 and encodes MCR-10 with 82.93% amino acids identical to MCR-9. mcr-10 confers 4-fold increase in colistin MIC (from 1 to 4 mg/L) when cloned into a colistin-susceptible E. roggenkampii strain. By screening GenBank, mcr-10 was found in various Enterobacteriaceae species of countries in four continents, suggesting that this gene has widely spread. MCR-10 shows 79.04% to 83.67% amino acid identity and highly conserved predicted protein structures with chromosomally encoded MCR-like phosphoethanolamine transferases (designated MCR-B here) of various Buttiauxella species. MCR-10, MCR-9 and MCR-B proteins may, therefore, originate from a common ancestor. mcr-10 was adjacent to a site-specific recombinase-encoding gene and was bracketed by IS903 and may be mobilized by site-specific recombination or composite transposon. Our results indicate that mcr-10 is a novel plasmid-borne colistin resistance gene and warrants immediate monitoring and further studies.