Cell-selective metabolic labeling of proteins
Cell-selective metabolic labeling of proteins
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DOI：
10.1038/nchembio.200
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发表时间：
2009-10-01
影响因子：
14.8
通讯作者：
Tirrell, David A.
中科院分区：
文献类型：
--
作者：
Ngo, John T.;Champion, Julie A.;Tirrell, David A.
用甲硫氨酸替代物叠氮正亮氨酸对蛋白质进行代谢标记可以通过突变型甲硫氨酰-tRNA合成酶（MetRS）的表达专门靶向特定细胞。在复杂的细胞混合物中，表达突变合成酶的细胞中产生的蛋白质可以用亲和试剂（用于检测或富集）或荧光染料（用于成像）标记。在不表达突变合成酶的细胞中产生的蛋白质既不被标记也不被检测到。
Metabolic labeling of proteins with the methionine surrogate azidonorleucine can be targeted exclusively to specified cells through expression of a mutant methionyl-tRNA synthetase (MetRS). In complex cellular mixtures, proteins made in cells that express the mutant synthetase can be tagged with affinity reagents (for detection or enrichment) or fluorescent dyes (for imaging). Proteins made in cells that do not express the mutant synthetase are neither labeled nor detected.