Blue light kills Aggregatibacter actinomycetemcomitans due to its endogenous photosensitizers
Blue light kills Aggregatibacter actinomycetemcomitans due to its endogenous photosensitizers
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DOI：
10.1007/s00784-013-1151-8
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发表时间：
2014-09-01
影响因子：
3.4
通讯作者：
Schmalz, Gottfried
中科院分区：
文献类型：
--
作者：
Cieplik, Fabian;Spaeth, Andreas;Schmalz, Gottfried
本研究的目的是证明牙周病原菌伴放线菌聚集杆菌（Aggregatibacteractinomycetemcomitans，AA）可以通过LED光固化装置产生的蓝光照射而被杀死。AA和大肠杆菌的浮游培养物用来自bluphaseA（R）C8光固化装置的蓝光照射，其发射峰位于460 nm，其通常应用于牙科树脂的聚合。进行CFU测定以分析处理后的活细菌。此外，裂解细菌细胞，裂解的AA和E.大肠杆菌的单线态氧的产生进行了研究。裂解的AA和E的光谱测量。结果表明，150 J/cm（2）的光剂量可使大肠杆菌的AA活性降低0.01%，而蓝光对大肠杆菌的AA活性没有影响。杆菌单重态氧发光的光谱分辨测量清楚地表明，在460 nm处激发时，从裂解的AA产生单重态氧信号。光谱测量裂解AA表现出的特征吸收和发射峰类似的那些已知的卟啉和flavin.AA可以被灭活的照射与蓝光，没有应用外源性photosensitize.These结果鼓励进一步研究的潜在用途，这些蓝光介导的自动光敏化过程中的牙周炎的治疗成功灭活Aggregatibacteractinomycetemcomitans。
The aim of this study was to demonstrate that the periodontal pathogen Aggregatibacter actinomycetemcomitans (AA) can be killed by irradiation with blue light derived from a LED light-curing unit due to its endogenous photosensitizers.Planktonic cultures of AA and Escherichia coli were irradiated with blue light from a bluephaseA (R) C8 light-curing unit with an emission peak at 460 nm, which is usually applied for polymerization of dental resins. A CFU-assay was performed for the analysis of viable bacteria after treatment. Moreover, bacterial cells were lysed and the lysed AA and E. coli were investigated for generation of singlet oxygen. Spectroscopic measurements of lysed AA and E. coli were performed and analyzed for characteristic absorption and emission peaks.A light dose of 150 J/cm(2) induced a reduction of a parts per thousand yen5 log(10) steps of viable AA, whereas no effect of blue light was found against E. coli. Spectrally resolved measurements of singlet oxygen luminescence showed clearly that a singlet oxygen signal is generated from lysed AA upon excitation at 460 nm. Spectroscopic measurements of lysed AA exhibited characteristic absorption and emission peaks similar to those of known porphyrins and flavins.AA can be inactivated by irradiation with blue light only, without application of an exogenous photosensitizer.These results encourage further studies on the potential use of these blue light-mediated auto-photosensitization processes in the treatment of periodontitis for the successful inactivation of Aggregatibacter actinomycetemcomitans.