Bacillus anthracis virulence in guinea pigs vaccinated with anthrax vaccine adsorbed is linked to plasmid quantities and clonality
Bacillus anthracis virulence in guinea pigs vaccinated with anthrax vaccine adsorbed is linked to plasmid quantities and clonality
复制标题
DOI：
10.1128/jcm.41.3.1212-1218.2003
复制
发表时间：
2003-03-01
影响因子：
9.4
通讯作者：
Hugh-Jones, ME
中科院分区：
文献类型：
--
作者：
Coker, PR;Smith, KL;Hugh-Jones, ME
炭疽芽孢杆菌是一种具有重要历史意义和现代意义的细菌性病原体。本研究收集了多项调查数据，表明炭疽芽孢杆菌的毒力与质粒pX01和pX02的克隆性和毒力有关。接种炭疽疫苗的豚鼠被20株具有世界范围遗传多样性代表性的炭疽芽孢杆菌感染。这些分离株的质粒拷贝数通过一种新的定量PCR方法进行了表征，该方法用于快速有效地检测环境样品中的炭疽芽孢杆菌。我们发现pX01和pX02的拷贝数与之前发表的报告不同。通过结合存活、质粒拷贝数和克隆的数据，我们开发了一个预测毒力的模型。该模型通过随机选择另外12株炭疽芽胞杆菌进行验证。这项研究的结果将有助于未来努力阐明这种重要病原体毒力变化的基础。
Bacillus anthracis is a bacterial pathogen of great importance, both historically and in the present. This study presents data collected from several investigations and indicates that B. anthracis virulence is associated with the clonality and virulence of plasmids pX01 and pX02. Guinea pigs vaccinated with Anthrax Vaccine Adsorbed were challenged with 20 B. anthracis isolates representative of worldwide genetic diversity. These same isolates were characterized with respect to plasmid copy number by using a novel method of quantitative PCR developed for rapid and efficient detection of B. anthracis from environmental samples. We found that the copy numbers for both pX01 and pX02 differed from those in previously published reports. By combining the data on survival, plasmid copy numbers, and clonality, we developed a model predicting virulence. This model was validated by using a randomly chosen set of 12 additional B. anthracis isolates. Results from this study will be helpful in future efforts to elucidate the basis for variation in the virulence of this important pathogen.