Djeyes absent (Djeya) controls prototypic planarian eye regeneration by cooperating with the transcription factor Djsix-1
Djeyes absent (Djeya) controls prototypic planarian eye regeneration by cooperating with the transcription factor Djsix-1
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DOI：
10.1016/j.ydbio.2004.01.042
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发表时间：
2004-05-13
影响因子：
2.7
通讯作者：
Batistoni, R
中科院分区：
文献类型：
--
作者：
Mannini, L;Rossi, L;Batistoni, R
在脊椎动物和无脊椎动物中，一个保守的核蛋白网络对眼睛的形成至关重要。淡水真涡虫可以再生眼睛没有Pax 6的贡献，这一发现表明，替代组合的调节元件可能控制的原型真涡虫眼睛的形态发生。为了进一步研究控制涡虫眼再生的分子事件，我们研究了日本三角涡虫眼缺失基因（Djeya）和6 -1基因（Djsix-1）的作用。这些基因在再生的眼睛和完整成人的分化的光感受器中表达。通过RNAi研究，我们发现Djsix-1和Djeya对于真涡虫正常眼睛的再生都是至关重要的，并且在体内进行遗传合作以建立正确的眼细胞分化。我们进一步证明了遗传相互作用是由这两种蛋白质的进化保守结构域之间的物理相互作用介导的。这些数据表明，Planarians合作使用Djsix-1和Djeya的光感受器的适当规范，这意味着涉及其进化保守域的机制可以是非常古老的。最后，Djsix-1和Djeya双链RNA在第二轮再生中产生无眼表型的效率更高。这可能是由于涡虫模型系统的显著可塑性，基于全能干细胞的稳定群体的存在，其确保所有分化细胞类型的快速细胞更新。(C)2004年爱思唯尔公司All rights reserved.
A conserved network of nuclear proteins is crucial to eye formation in both vertebrates and invertebrates. The finding that freshwater planarians can regenerate eyes without the contribution of Pax6 suggests that alternative combinations of regulatory elements may control the morphogenesis of the prototypic planarian eye. To further dissect the molecular events controlling eye regeneration in planarians, we investigated the role of eyes absent (Djeya) and six-1 (Djsix-1) genes in Dugesia japonica. These genes are expressed in both regenerating eyes and in differentiated photoreceptors of intact adults. Through RNAi studies, we show that Djsix-1 and Djeya are both critical for the regeneration of normal eyes in planarians and genetically cooperate in vivo to establish correct eye cell differentiation. We further demonstrate that the genetic interaction is mediated by physical interaction between the evolutionarily conserved domains of these two proteins. These data indicate that planarians use cooperatively Djsix-1 and Djeya for the proper specification of photoreceptors, implicating that the mechanism involving their evolutionarily conserved domains can be very ancient. Finally, both Djsix-1 and Djeya double-stranded RNA are substantially more effective at producing no-eye phenotypes in the second round of regeneration. This is probably due to the significant plasticity of the planarian model system, based on the presence of a stable population of totipotent stem cells, which ensure the rapid cell turnover of all differentiated cell types. (C) 2004 Elsevier Inc. All rights reserved.