GLUCOCORTICOID REGULATION OF PREPROENKEPHALIN GENE-EXPRESSION IN THE RAT FOREBRAIN
GLUCOCORTICOID REGULATION OF PREPROENKEPHALIN GENE-EXPRESSION IN THE RAT FOREBRAIN
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DOI：
10.1016/0169-328x(92)90201-l
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发表时间：
1992-11-01
期刊：
影响因子：
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通讯作者：
HARLAN, RE
中科院分区：
文献类型：
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作者：
AHIMA, RS;GARCIA, MM;HARLAN, RE
应用原位杂交和斑点杂交技术研究了糖皮质激素对大鼠前脑前脑啡肽原（PPE）mRNA水平的影响。在肾上腺完整的大鼠，PPE mRNA的高水平，原位杂交评估，位于尾壳核，核杏仁核，中央杏仁核，和腹外侧腹内侧下丘脑（VMHVL），在海马和低水平。肾上腺切除后，除海马外，其余各区域PPE mRNA阳性细胞密度和PPE mRNA/细胞水平均降低。皮质酮（CORT）的急性治疗肾上腺切除大鼠增加PPE mRNA/细胞的水平在尾壳核和VMHVL。在完整大鼠中，CORT慢性治疗增加了尾壳核和海马中PPE mRNA阳性细胞的密度，以及尾壳核和丘脑核中PPE mRNA/细胞的水平。用斑点印迹法评估慢性CORT处理对纹状体、杏仁核、海马和下丘脑内侧基底核PPE mRNA的影响。慢性CORT治疗增加PPE mRNA水平在尾壳核和海马。从斑点杂交和原位杂交中获得的结果表明，慢性CORT治疗对完整大鼠PPE mRNA水平的影响具有良好的相关性。这项研究的结果表明，糖皮质激素的基础PPE mRNA水平的维持所需的大部分地区的大鼠前脑。然而，肾上腺切除和完整大鼠中糖皮质激素治疗对PPE mRNA水平的影响存在相当大的区域异质性。PPE mRNA水平的增加响应于高循环水平的糖皮质激素，例如在应激中，可能具有重要的病理生理学后果。
The effects Of glucocorticoids on the levels of preproenkephalin (PPE) mRNA in the rat forebrain were analyzed with in situ hybridization and dot blots. In adrenally-intact rats, high levels of PPE mRNA, as assessed by in situ hybridization, were localized in the caudate-putamen, nucleus accumbens, central amygdala, and ventrolateral ventromedial hypothalamus (VMHVL), and low levels in the hippocampus. After adrenalectomy, the density of PPE mRNA-positive cells and the level of PPE mRNA/cell were decreased in all regions except the hippocampus. Acute treatment with corticosterone (CORT) in adrenalectomized rats increased the level of PPE mRNA/cell in the caudate-putamen and VMHVL. In intact rats, chronic treatment with CORT increased the density of PPE mRNA-positive cells in the caudate-putamen and hippocampus, and the level of PPE mRNA/cell in the caudate-putamen and nucleus accumbens. The effect of chronic CORT treatment on PPE mRNA in the striatum, amygdala, hippocampus and mediobasal hypothalamus was assessed with dot blots. Chronic CORT treatment increased PPE mRNA levels in the caudate-putamen and hippocampus. There was a good correlation between results on the effect of chronic CORT treatment on PPE mRNA levels in intact rats, obtained from dot blots and in situ hybridization. Results from this study suggest that glucocorticoids are required for the maintenance of basal PPE mRNA levels in most regions of the rat forebrain. There is, however, considerable regional heterogeneity in the effect of glucocorticoid treatment on PPE mRNA levels in adrenalectomized and intact rats. Increased PPE mRNA levels in response to high circulating levels of glucocorticoids, e.g. in stress, may have important pathophysiological consequences.