Combined atomic force microscopy and side-view optical imaging for mechanical studies of cells.
Combined atomic force microscopy and side-view optical imaging for mechanical studies of cells.
复制标题
原子力显微镜和侧视光学成像，用于细胞的机械研究。
DOI：
10.1038/nmeth.1320
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发表时间：
2009-05
期刊：
影响因子：
48
通讯作者：
Fletcher, Daniel A.
中科院分区：
文献类型：
--
作者：
Chaudhuri, Ovijit;Parekh, Sapun H.;Lam, Wilbur A.;Fletcher, Daniel A.
The mechanical rigidity of cells and adhesion forces between cells are important in various biological processes, including cell differentiation, proliferation and tissue organization. Atomic force microscopy has emerged as a powerful tool to quantify the mechanical properties of individual cells and adhesion forces between cells. Here we demonstrate an instrument that combines atomic force microscopy with a side-view fluorescent imaging path that enables direct imaging of cellular deformation and cytoskeletal rearrangements along the axis of loading. With this instrument, we directly observed cell shape under mechanical load, correlated changes in shape with force-induced ruptures and imaged formation of membrane tethers during cell-cell adhesion measurements. Additionally, we observed cytoskeletal reorganization and stress-fiber formation while measuring the contractile force of an individual cell. This instrument can be a useful tool for understanding the role of mechanics in biological processes.
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影响因子：
3.4
作者：
Charras, GT;Horton, MA
通讯作者：
Horton, MA
影响因子：
3.4
作者：
Alcaraz, J;Buscemi, L;Navajas, D
通讯作者：
Navajas, D
影响因子：
21.3
作者：
Parekh, SH;Chaudhuri, O;Fletcher, DA
通讯作者：
Fletcher, DA
影响因子：
3.4
作者：
Bausch, AR;Möller, W;Sackmann, E
通讯作者：
Sackmann, E
DOI：
10.1083/jcb.200403043
发表时间：
2004-12-20
期刊：
The Journal of cell biology
影响因子：
--
作者：
Chu YS;Thomas WA;Eder O;Pincet F;Perez E;Thiery JP;Dufour S
通讯作者：
Dufour S