Evidence for limited species diversity of bacteriochlorophyll b-containing purple nonsulfur anoxygenic phototrophs in freshwater habitats
Evidence for limited species diversity of bacteriochlorophyll b-containing purple nonsulfur anoxygenic phototrophs in freshwater habitats
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DOI：
10.1016/s0378-1097(02)01195-3
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发表时间：
2003-01-28
影响因子：
2.1
通讯作者：
Madigan, MT
中科院分区：
文献类型：
--
作者：
Hoogewerf, GJ;Jung, DO;Madigan, MT
采用长波滤光剂和消光稀释等特定富集方法，从4种淡水生境中分离出13株含叶绿素b的紫色非硫细菌。将新分离菌株与绿芽绿藻（Blastochloris viridis）型菌株DSM 133(T)在色素、形态、碳营养和系统发育方面进行了比较。所有新分离株均为芽殖菌，光养群体培养物颜色为绿色、棕色或棕绿色。各菌株光分解碳源模式相似；然而，单糖和双糖被新分离株广泛使用，而它们不支持菌株DSM 133(T)的生长。系统发育分析表明，除1株棕色菌株和1株轻度嗜热菌株外，所有新菌株均与型菌株紧密聚类。结果表明，与含有细菌叶绿素a的紫色非硫细菌相比，含有细菌叶绿素b的紫色非硫细菌可能在形态或系统发育上没有多样性，并与所有其他无氧光养生物归为一个紧密的系统发育分支。(C) 2003欧洲微生物学会联合会。Elsevier Science B.V.版权所有。
Thirteen new isolates of bacteriochlorophyll b-containing purple nonsulfur bacteria were isolated from four freshwater habitats using specific enrichment methods including the use of long wavelength filters and extincting dilution of the inoculum. The new isolates were compared with the type strain of Blastochloris viridis, strain DSM 133(T), as regards pigments, morphology, carbon nutrition, and phylogeny. All new isolates were budding bacteria, and phototrophic mass cultures were green, brown, or brown-green in color. The pattern of carbon sources photocatabolized were similar in all strains; however, sugars, both mono- and disaccharides, were widely used by the new isolates while they did not support growth of strain DSM 133(T). Phylogenetic analysis showed all new strains to cluster tightly with the type strain with the exception of one brown-colored strain and a mildly thermophilic strain. The results suggest that in contrast to purple nonsulfur bacteria containing bacteriochlorophyll a, those containing bacteriochlorophyll b may not be morphologically or phylogenetically diverse, and group into a tight phylogenetic clade distinct from all other anoxygenic phototrophs. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.