Aberrant SERCA3 expression is closely linked to pathogenesis, invasion, metastasis, and prognosis of gastric carcinomas
Aberrant SERCA3 expression is closely linked to pathogenesis, invasion, metastasis, and prognosis of gastric carcinomas
复制标题
SERCA3异常表达与胃癌的发病、侵袭、转移及预后密切相关
DOI：
10.1007/s13277-012-0444-x
复制
发表时间：
2012-09
期刊：
影响因子：
--
通讯作者：
Zheng, Hua-chuan
中科院分区：
文献类型：
--
作者：
Yu, Miao;Mao, Xiao-yun;Takano, Yasuo;Zheng, Hua-chuan
Sarco (endo)plasmic reticulum Ca2+-ATPase (SERCAs) 3 is involved in calcium mobilization from endoplasmic reticulum into cytosol and closely links to metabolism, neuronal plasticity, gene transcription, cell growth, differentiation, apoptosis, protein folding, and carcinogenesis. To clarify the role ofSERCA3in gastric carcinogenesis and subsequent progression, its expression was examined by immunohistochemistry and in situ hybridization (ISH) on tissue microarrays containing gastric carcinomas, adjacent non-neoplastic mucosa (NNM), and metastatic lymph node.SERCA3expression was studied in gastric carcinoma tissue and cell lines by Western blot, reverse transcriptase-polymerase chain reaction, or immunofluorescence. The results demonstrated thatSERCA3was distinctively expressed in GES-1, AGS, BGC-823, GT-3TKB, HGC-27, KATO-III, MGC-803, MKN28, MKN45, SCH, SGC-7901, and STKM-2 at both mRNA and protein levels. The carcinomas showed higherSERCA3mRNA expression than the matched NNM by real-time PCR and ISH (P< 0.05). Immunohistochemically, SERCA3 expression was decreased from gastric NNM, primary to metastatic carcinoma (P< 0.05). SERCA3 expression was negatively related to depth of invasion, distant metastasis, and tumor node metastasis (TNM) staging (P< 0.05), but not to age, sex, lymphatic or venous invasion, or lymph node metastasis (P> 0.05). Kaplan–Meier analysis indicated that SERCA3 expression was positively associated with favorable prognosis of the patients with gastric carcinoma (P< 0.05). Cox’s proportional hazard model indicated that venous invasion, distant metastasis and TNM staging (P< 0.05) were independent prognostic factors for gastric carcinomas. It was suggested that downregulated SERCA3 expression is closely linked to pathogenesis, invasion, metastasis, and prognosis of gastric carcinomas. It might be employed to indicate the pathobiological behaviors and prognosis of gastric carcinomas.
登录
查看更多内容
DOI：
10.1016/0169-328x(96)00088-5
发表时间：
1996-09-05
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
--
作者：
BabaAissa, F;Raeymaekers, L;Casteels, R
通讯作者：
Casteels, R
影响因子：
--
作者：
A. Hovnanian
通讯作者：
A. Hovnanian
影响因子：
3.4
作者：
Zheng, Huachuan;Takahashi, Hiroyuki;Takano, Yasuo
通讯作者：
Takano, Yasuo
DOI：
--
发表时间：
1994-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
M. Roe;L. Philipson;C. Frangakis;A. Kuznetsov;R. Mertz;M. E. Lancaster;B. Spencer;J. Worley;I. Dukes
通讯作者：
M. Roe;L. Philipson;C. Frangakis;A. Kuznetsov;R. Mertz;M. E. Lancaster;B. Spencer;J. Worley;I. Dukes
DOI：
--
发表时间：
2009
期刊：
--
影响因子：
--
作者：
通讯作者：
--