LidA, a translocated substrate of the Legionella pneumophila type IV secretion system, interferes with the early secretory pathway
LidA, a translocated substrate of the Legionella pneumophila type IV secretion system, interferes with the early secretory pathway
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DOI：
10.1128/iai.73.7.4370-4380.2005
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发表时间：
2005-07-01
影响因子：
3.1
通讯作者：
Isberg, RR
中科院分区：
文献类型：
--
作者：
Derré, I;Isberg, RR
嗜肺军团菌使用IV型分泌系统将效应分子递送到宿主细胞中并建立其复制泡。在这项研究中，我们研究了LidA的作用，LidA是一种与L.含嗜肺细胞的空泡。利达在细菌的整个复制周期中分泌到宿主细胞中，并与早期分泌途径的隔室相关联。当在哺乳动物细胞或酵母中过表达时，利达可能通过预测富含卷曲螺旋结构的结构域干扰早期分泌途径。最后，在细胞内复制过程中，复制空泡与内质网-高尔基体中室和高尔基体密切接触，表明细胞内生长与空泡与早期分泌途径的室之间呈正相关。我们认为利达参与了早期分泌囊泡向L.嗜肺细胞的空泡和空泡与分泌途径，以促进这一进程。
Legionella pneumophila uses a type IV secretion system to deliver effector molecules into the host cell and establish its replication vacuole. In this study, we investigated the role of LidA, a translocated substrate associated with the surface of the L. pneumophila-containing vacuole. LidA is secreted into the host cell throughout the replication cycle of the bacteria and associates with compartments of the early secretory pathway. When overexpressed in mammalian cells or yeast, LidA interferes with the early secretory pathway, probably via a domain predicted to be rich in coiled-coil structure. Finally, during intracellular replication, the replication vacuoles are in close contact with the endoplasmic reticulum-Golgi intermediate compartment and the Golgi apparatus, suggesting a positive correlation between intracellular growth and association of the vacuole with compartments of the early secretory pathway. We propose that LidA is involved in the recruitment of early secretory vesicles to the L. pneumophila-containing vacuole and that the vacuole associates with the secretory pathway to facilitate this process.