The Arabidopsis AMP1 Gene Encodes a Putative Glutamate Carboxypeptidase
The Arabidopsis AMP1 Gene Encodes a Putative Glutamate Carboxypeptidase
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DOI：
10.1105/tpc.010146
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发表时间：
2001-09
期刊：
影响因子：
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通讯作者：
C. Helliwell;A. N. Chin-Atkins;I. Wilson;R. Chapple;E. Dennis;A. Chaudhury
中科院分区：
文献类型：
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作者：
C. Helliwell;A. N. Chin-Atkins;I. Wilson;R. Chapple;E. Dennis;A. Chaudhury
拟南芥amp 1突变体显示多效性表型，包括改变芽顶端分生组织，增加细胞增殖，多子叶，组成型光形态建成，开花时间早，内源性细胞分裂素水平增加，细胞周期蛋白cycD 3表达增加。通过图位克隆的方法，我们分离出了AMP 1基因。AMP 1 cDNA编码706个氨基酸的多肽，与谷氨酸羧肽酶具有显著的相似性。AMP 1 mRNA在所有组织中均有表达，在根、茎、花序和角果中表达较高。微阵列分析确定了四种mRNA的表达改变的两个等位基因的amp 1，包括上调CYP 78 A5，这已被证明是标志着拍摄顶端分生组织的边界。AMP 1蛋白与谷氨酸羧肽酶，特别是与N-乙酰基α-连接的酸性二肽酶的相似性表明AMP 1基因产物调节小信号分子的水平，该小信号分子用于调节植物发育的许多方面，特别是顶端分生组织的大小。
Arabidopsis amp1 mutants show pleiotropic phenotypes, including altered shoot apical meristems, increased cell proliferation, polycotyly, constitutive photomorphogenesis, early flowering time, increased levels of endogenous cytokinin, and increased cyclin cycD3 expression. We have isolated the AMP1 gene by map-based cloning. The AMP1 cDNA encodes a 706;–amino acid polypeptide with significant similarity to glutamate carboxypeptidases. The AMP1 mRNA was expressed in all tissues examined, with higher expression in roots, stems, inflorescences, and siliques. Microarray analysis identified four mRNA species with altered expression in two alleles of amp1, including upregulation of CYP78A5, which has been shown to mark the shoot apical meristem boundary. The similarity of the AMP1 protein to glutamate carboxypeptidases, and in particular to N-acetyl α-linked acidic dipeptidases, suggests that the AMP1 gene product modulates the level of a small signaling molecule that acts to regulate a number of aspects of plant development, in particular the size of the apical meristem.